Role of phospholipid in the low affinity reactions between cytochrome c and cytochrome oxidase.
Role of phospholipid in the low affinity reactions between cytochrome c and cytochrome oxidase.
复制标题
磷脂在细胞色素 c 和细胞色素氧化酶之间的低亲和力反应中的作用。
DOI：
10.1016/0014-5793(83)80321-4
复制
发表时间：
1983
期刊：
影响因子：
3.5
通讯作者：
Margoliash,E
中科院分区：
文献类型：
--
作者：
Speck,SH;Neu,CA;Swanson,MS;Margoliash,E
通过分光光度法监测的细胞色素氧化酶对亚铁细胞色素的稳态氧化表明：（1）纯化酶的动力学严格为双相，而线粒体膜结合酶则表现出具有延长的低亲和力相的多相动力学； (2) 两种制剂的最高亲和力相的 TNmax 慢至 5-10 电子·s−1，而对于低亲和力相，纯化酶约为 45 电子·s−1，线粒体膜结合酶约为 150 电子·s−1； (3)将纯化的酶重构到酸性磷脂囊泡中部分地填充了延长的低亲和力相，而重构到不带电的囊泡中则没有效果。
The steady‐state oxidation of ferrocytochromecby cytochrome oxidase monitored spectrophotometrically showed that: (1) the kinetics were strictly biphasic with purified enzyme, while mitochondrial membrane‐bound enzyme exhibited multiphasic kinetics with extended low affinity phases; (2) theTNmaxfor the highest affinity phase was as slow as 5–10 electron · s−1for both preparations, while for the low affinity phases it was about 45 electron · s−1for the purified enzyme and 150 electron · s−1for the mitochondrial membrane‐bound enzyme; (3) reconstitution of purified enzyme into acidic phospholipid vesicles partially repleted the extended low affinity phases, while reconstitution into uncharged vesicles had no effect.