NEW BIOCHEMICAL MARKER FOR BONE METABOLISM - MEASUREMENT BY RADIOIMMUNOASSAY OF BONE GLA PROTEIN IN THE PLASMA OF NORMAL SUBJECTS AND PATIENTS WITH BONE-DISEASE
NEW BIOCHEMICAL MARKER FOR BONE METABOLISM - MEASUREMENT BY RADIOIMMUNOASSAY OF BONE GLA PROTEIN IN THE PLASMA OF NORMAL SUBJECTS AND PATIENTS WITH BONE-DISEASE
复制标题
DOI：
10.1172/jci109954
复制
发表时间：
1980-01-01
影响因子：
15.9
通讯作者：
NISHIMOTO, SK
中科院分区：
文献类型：
--
作者：
PRICE, PA;PARTHEMORE, JG;NISHIMOTO, SK
γ-羧基谷氨酸含骨蛋白(BGP)是哺乳动物骨骼中含量丰富的非胶原蛋白。BGP的相对分子质量为5800，含有3个残基，即依赖维生素K的氨基酸--γ-羧基谷氨酸。用放射免疫法测定了109例正常人和112例各种骨病患者血浆中骨钙素的含量。人血浆中的骨钙素与小牛骨钙素经稀释法和凝胶渗透层析法检测无明显差别。平均值(.+-.正常人骨钙素浓度为6.78(.+-)。0.20)ng/ml，7.89(.+-.男性为0.32)，男性为4.85(+-)。女性为0.35)。骨钙素在Paget‘S病、骨转移瘤、原发性甲状旁腺功能亢进症、肾性骨营养不良和骨量减少的患者中升高。血浆骨钙素与血浆碱性磷酸酶(AP)在某些情况下确实相关，但两者之间存在分离。肝病患者血浆BGP正常，但AP升高，反映AP对骨病缺乏特异性。血浆BGP放射免疫法可作为骨病患者的一种有用而特异的方法。
.gamma.-Carboxyglutamic acid-containing protein of bone (BGP) is an abundant noncollagenous protein of mammalian bone. BGP has an MW of 5800 and contains 3 residues of the vitamin K-dependent amino acid, .gamma.-carboxyglutamic acid. A radioimmunoassay based on calf BGP was applied for the measurement of the protein in the plasma of 109 normal humans and 112 patients with various bone diseases. BGP in human plasma was indistinguishable from calf BGP by assay dilution studies and gel permeation chromatography. The mean (.+-. SE) concentration of BGP in normal subjects was 6.78 (.+-. 0.20) ng/ml, 7.89 (.+-. 0.32) for males and 4.85 (.+-. 0.35) for females. Plasma BGP was increased in patients with Paget''s disease of bone, bone metastases, primary hyperparathyroidism, renal osteodystrophy and osteopenia. Plasma BGP did correlate with plasma alkaline phosphatase (AP) in some instances, but there were dissociations between the two. Patients with liver disease had normal plasma BGP despite increased plasma AP, a reflection of the lack of specificity of AP measurements for bone disease. Radioimmunoassay of plasma BGP can be a useful and specific procedure for evaluating the patient with bone disease.