Antibodies anti bloodstream and circumsporozoite antigens (Plasmodium vivax and Plasmodium malariae P-brasilianum) in areas of very low malaria endemicity in Brazil
Antibodies anti bloodstream and circumsporozoite antigens (Plasmodium vivax and Plasmodium malariae P-brasilianum) in areas of very low malaria endemicity in Brazil
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DOI：
10.1590/s0074-02761997000200017
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发表时间：
1997-03-01
影响因子：
2.8
通讯作者：
Kloetzel, JK
中科院分区：
文献类型：
--
作者：
Curado, I;Duarte, AMRC;Kloetzel, JK
1992-1994年期间，在巴西的两个地区报告了33例疟疾病例，这些地区很少发生散发的非典型病例，其中大多数发生在周末度假的房主身上，而家庭看护者则长期居住在那里。间日疟原虫间接荧光抗体检测（IFAT），以及3种已知间日疟原虫和疟疾疟原虫的环孢子子（CS）蛋白重复肽酶联免疫吸附试验（ELISA）。对在疟疾病例5至10公里范围内获得的277份血清进行了Brasilianum检测。这些捐助者很少回忆起典型的疟疾发作。除5人外，其余人的血涂片均呈阴性。血清学中包含的5例疟疾病例中有1例是房主，1例是常住居民，3例是地方病控制中心的雇员他们去那里抓蚊子。在1区，IFAT阳性血清的患病率在看护者中分别为73%和28%，在房主中分别为18%和9.6%。在区域2（3个地方），看护者和房主之间没有区别，IFAT阳性率分别为38%、28%和7%。ELISA检测的抗- cs重复序列的相对阳性百分率在不同的位点上有所不同。住所在树林附近，那里经常可以看到猴子。讨论了这些疟疾病例的来源、地理差异和高血清阳性率。
During 1992-1994, 33 malaria cases were reported in two regions in Brazil where few sporadic atypical cases occur most of them in home owners, who are weekenders, while home caretakers live there permanently. Indirect Fluorescent Antibody Test (IFAT), with Plasmodium vivax, and Enzime Linked Immunosorbent Assay (ELISA) with repeat peptides of the circumsporozoite (CS) proteins of the 3 known P. vivax variants and P. malarie/P. brasilianum, were performed on 277 sera, obtained within a 5 to 10 km range of malaria cases. Very rarely did any of these donors recall typical malaria episodes. Blood smears of all but 5 were negative. One of the 5 malaria cases included in our serology was of a home owner, 1 of a permanent resident, 3 from Superintendencia de Controle de Endemias employees who went there to capture mosquitoes. In Region 1 the prevalence of IFAT positive sera was 73% and 28% among caretakers, 18% and 9.6% among home owners. In Region 2 (3 localities) no distinction was possible between caretakers and home owners, IFAT positivity being 38%, 28% and 7%. The relative percentage of positive anti-CS repeats ELISA, differed for each of the peptides among localities. Dwellings are in the vicinity of woods, where monkeys are frequently seen. The origin of these malaria cases, geographical differences and high seropositivity is discussed.