High Mobility Group Box 1 (HMGB1) Levels in the Placenta and in Serum in Preeclampsia
High Mobility Group Box 1 (HMGB1) Levels in the Placenta and in Serum in Preeclampsia
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DOI：
10.1111/j.1600-0897.2010.00975.x
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发表时间：
2011-08-01
影响因子：
3.6
通讯作者：
Taketani, Yuji
中科院分区：
文献类型：
--
作者：
Wang, Bo;Koga, Kaori;Taketani, Yuji
先兆子痫是一种以全身炎症为特征的妊娠期疾病。高迁移率族蛋白1（HMGB 1）是一种已知的分子，通过参与各种炎症过程作为“危险信号”，但关于先兆子痫的数据很少。本研究的目的是分析胎盘和血清HMGB 1水平在正常妊娠和先兆子痫。研究方法血清收集先兆子痫的妇女后不久，疾病的表现和开始任何药物治疗前。分娩后立即收集胎盘样品。通过Western印迹分析评估胎盘中HMGB 1（28-和30-kDa）的表达亚型。血清HMGB 1浓度测定使用酶联免疫吸附试验（ELISA）。结果HMGB 1的两种异构体表达的人胎盘。28-和30-kDa HMGB 1亚型在先兆子痫胎盘组织中高度表达;然而，与正常血压对照组织相比，检测到的表达水平差异未达到统计学显著性。对照组和子痫前期组血清HMGB 1水平无显著性差异。结论HMGB 1引起的炎症反应可能参与子痫前期胎盘的促炎过程。需要进一步的研究来阐明HMGB 1在先兆子痫中的确切作用。
ProblemPreeclampsia is a pregnancy disorder characterized by systemic inflammation. High mobility group box 1 (HMGB1) is a molecule known to act as a 'danger signal' by participating in various inflammatory processes, but data in regard to preeclampsia are sparse. The aim of this study was to analyze placental and serum HMGB1 levels in normal pregnancy and preeclampsia.Method of studySera were collected from women with preeclampsia soon after the manifestation of the disease and before commencing any medication. Placental samples were collected immediately after delivery. Expressed isoforms of HMGB1 (28- and 30-kDa) in the placenta were evaluated by Western blot analysis. Serum HMGB1 concentrations were measured using enzyme-linked immunosorbent assays (ELISA).ResultsTwo isoforms of HMGB1 are expressed by the human placenta. The 28- and 30-kDa HMGB1 isoforms were expressed highly in preeclamptic placental tissue; however, compared with normotensive control tissue, differences in detected expression levels did not reach statistical significance. No significant difference was observed in serum HMGB1 levels between control and preeclampsia.ConclusionInflammation provoked by HMGB1 is likely to be involved in the proinflammatory process in preeclamptic placenta. Further studies are needed to elucidate the precise role of HMGB1 in preeclampsia.