EPFL peptide signaling ensures robust self‐pollination success under cool temperature stress by aligning the length of the stamen and pistil.
EPFL peptide signaling ensures robust self‐pollination success under cool temperature stress by aligning the length of the stamen and pistil.
复制标题
EPFL 肽信号通过调整雄蕊和雌蕊的长度，确保在低温胁迫下实现稳健的自花授粉成功。
DOI：
10.1111/pce.14498
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Uchida N
中科院分区：
文献类型：
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作者：
Negoro S;Hirabayashi T;Iwasaki R;Torii KU;Uchida N
植物成功的有性繁殖需要对温度敏感的过程，而温度胁迫有时会导致雄性和雌性生殖组织之间的发育不同步。在拟南芥中，当雄蕊和雌蕊的长度排成一朵花，雄蕊顶端的花粉传递到雌蕊顶端的柱头时，就会发生自花授粉。尽管细胞间信号在几个生殖步骤中起作用，但信号分子，包括分泌肽，如何促进生殖组织的同步生长仍然有限。在这里，我们发现了分泌肽EPIDERMAL模式因子样6(EPFL6)的突变体，它在中温下没有表现出表型，但在寒冷的温度下，由于雄蕊伸长不足而导致果实生产失败。EPFL6在雄蕊花丝中表达，并促进花丝伸长，在寒冷的温度下实现雄蕊和雌蕊长度的排列。我们还发现，在中等温度下，所有EPFL6亚家族基因都是雄蕊伸长所必需的。此外，我们还发现ERECTA(ER)作为EPFL家族多肽的共同受体，调节雄蕊-雌蕊的生长协调。最后，我们提供了证据表明，ER活性的调节通过重新排列雄蕊和雌蕊长度来挽救由于雄蕊伸长不足而导致的生殖失败。
Successful sexual reproduction of plants requires temperature‐sensitive processes, and temperature stress sometimes causes developmental asynchrony between male and female reproductive tissues. InArabidopsis thaliana, self‐pollination occurs when the stamen and pistil lengths are aligned in a single flower so that pollens at the stamen tip are delivered to the stigma at the pistil tip. Although intercellular signalling acts in several reproduction steps, how signalling molecules, including secreted peptides, contribute to the synchronous growth of reproductive tissues remains limited. Here, we show that the mutant of the secreted peptideEPIDERMAL PATTERNING FACTOR LIKE 6(EPFL6), which shows no phenotypes at a moderate temperature, fails in fruit production at a cool temperature due to insufficient elongation of stamens.EPFL6is expressed in stamen filaments and promotes filament elongation to achieve the alignment of stamen and pistil lengths at a cool temperature. We also found that, at a moderate temperature, allEPFL6‐subfamily genes are required for stamen elongation. Furthermore, we showed that ERECTA (ER), known as a common receptor for EPFL‐family peptides, mediates the stamen–pistil growth coordination. Lastly, we provided evidence that modulation of ER activity rescues the reproduction failure caused by insufficient stamen elongation by realigning the stamen and pistil lengths.