Analysis of expressed sequence tags of the water flea Daphnia magna
Analysis of expressed sequence tags of the water flea Daphnia magna
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作者：
Watanabe, H;Tatarazako, N;Iguchi, T
为研究大型蚤基因表达，我们构建了cDNA文库，并对7210个克隆的表达序列标签（EST）进行了分析。EST序列聚为2958个非冗余组。对蛋白质和DNA数据库的BLAST分析显示，1218个（41%）独特序列与已知的核苷酸或氨基酸序列具有显着相似性，而其余1740个（59%）与其他基因没有显着相似性。聚类分析显示，特别高的表达与ATP合成，结构蛋白和蛋白酶的基因。这些cDNA克隆和EST序列信息将为进一步研究水蚤的生物学功能以及生态学与基因组学之间的联系提供有用的信息。
To study gene expression in the water flea Daphnia magna we constructed a cDNA library and characterized the expressed sequence tags (ESTs) of 7210 clones. The EST sequences clustered into 2958 nonredundant groups. BLAST analyses of both protein and DNA databases showed that 1218 (41%) of the unique sequences shared significant similarities to known nucleotide or amino acid sequences, whereas the remaining 1740 (59%) showed no significant similarities to other genes. Clustering analysis revealed particularly high expression of genes related to ATP synthesis, structural proteins, and proteases. The cDNA clones and EST sequence information should be useful for future functional analysis of daphnid biology and investigation of the links between ecology and genomics.