(H-3)-LABELED MUSCIMOL, (H-3)-LABELED NIPECOTIC ACID AND (H-3)-LABELED ISOGUVACINE AS AUTORADIOGRAPHIC MARKERS FOR GABA NEUROTRANSMISSION
(H-3)-LABELED MUSCIMOL, (H-3)-LABELED NIPECOTIC ACID AND (H-3)-LABELED ISOGUVACINE AS AUTORADIOGRAPHIC MARKERS FOR GABA NEUROTRANSMISSION
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DOI：
10.1007/bf01249274
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发表时间：
1982-01-01
影响因子：
3.3
通讯作者：
EHINGER, B
中科院分区：
文献类型：
--
作者：
AGARDH, E;EHINGER, B
将家兔、金鱼和豚鼠的视网膜分别暴露于（3H）标记的GABA、muscimol、nipecotic acid和异鸟碱中，在体内通过玻璃体内注射或在平衡盐溶液中孵育。然后用放射自显影法研究3H的分布。所有的物质都标记了一组类似的假定的无毛细胞。0度孵育。C，在10-5 M沃巴因或10-3 M GABA中，抑制(3H)-muscimol的标记，而双库兰（10-4 M）和甘氨酸（10-3 M）是效率较低的阻滞剂。结果被解释为神经元摄取(3H)-muscimol而不是GABA受体结合。除了(3H)-异guvacine外，所有的物质都能标记神经胶质细胞，以至于在兔子和金鱼身上，神经元标记往往是伪装的。在豚鼠中，muscimol对胶质细胞的标记不太明显。（3H）-异guvacine（仅在家兔中测试）对GABA神经元分布的细胞有很强的标记作用，而对胶质细胞的标记作用很小。
Retinas from rabbit, goldfish and guinea pig were exposed to (3H)-labeled GABA, muscimol, nipecotic acid and isoguvaine either by intravitreal injection in vivo or by incubations in a balanced salt solution. The distribution of 3H was then studied with autoradiography. All substances labeled a similar set of presumed amacrine cells. Incubating at 0.degree. C, in 10-5 M ouabain or 10-3 M GABA, inhibited the labeling by (3H)-muscimol, whereas bicuculline (10-4 M) and glycine (10-3 M) were less efficient blockers. The result is interpreted as a neuronal uptake of (3H)-muscimol rather than as a GABA receptor binding. All the substances except (3H)-isoguvacine also labeled glia to such a degree that neuronal labeling was often disguised in rabbits and goldfish. Glial labeling by muscimol was less pronounced in the guinea pig. (3H)-isoguvacine (tested only in rabbits) gave a strong labeling of cells with the distribution of GABA neurons and only little glial labeling.