Comamonas zonglianii sp. nov., isolated from phenol-contaminated soil.
Comamonas zonglianii sp. nov., isolated from phenol-contaminated soil.
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DOI：
10.1099/ijs.0.019612-0
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发表时间：
2011-02
影响因子：
2.8
通讯作者：
Xin-Yan Yu;Yong-Feng Li;Jin-wei Zheng;Yi Li;Lian-tai Li;Jian He;Shun-Peng Li
中科院分区：
文献类型：
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作者：
Xin-Yan Yu;Yong-Feng Li;Jin-wei Zheng;Yi Li;Lian-tai Li;Jian He;Shun-Peng Li
从苯酚污染土壤中分离到一株细菌BF-3（T），并对其进行了多相分类研究。细胞为革兰氏反应阴性、非孢子形成、非运动、短杆状。基于16 S rRNA基因序列的系统发育分析表明，BF-3（T）菌株在丛毛单胞菌属的进化辐射边缘形成了一个单系分支，与水生丛毛单胞菌LMG 2370（T）、C. nitratiovorans DSM 13191（T）（96.4%），C.齿白蚁LMG 23579（T）（96.4%）; kerstersii LMG 3475（T）（96.3%），C. koreensis KCTC 12005（T）和C. terrigena LMG 1253（T）（96.0%）。主要的细胞脂肪酸是C（16：0）、C（18：1）/C（18：1）ω 7 c、C（17：0）环和总和特征3（C（16：1）ω 7 c和/或iso-C（15：0）2-OH）。根据系统发育分析、DNA-DNA杂交、全细胞脂肪酸组成和生化特性，菌株BF-3（T）与其他已知的丛毛单胞菌属的模式菌株明显不同，因此，菌株BF-3（T）代表丛毛单胞菌属的一个新种，建议将其命名为丛毛单胞菌（Comamonaszonglianii sp. nov.）。模式菌株为BF-3（T）（=CCTCC AB 209170（T）=DSM 22523（T））。
A bacterial strain, designated BF-3(T), was isolated from phenol-contaminated soil and investigated using a polyphasic taxonomic approach. Cells were Gram-reaction-negative, non-sporulating, non-motile, short rods. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BF-3(T) formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Comamonas; it showed highest sequence similarities to Comamonas aquatica LMG 2370(T) (96.8 %), C. nitrativorans DSM 13191(T) (96.4 %), C. odontotermitis LMG 23579(T) (96.4 %), C. kerstersii LMG 3475(T) (96.3 %), C. koreensis KCTC 12005(T) (96.1 %) and C. terrigena LMG 1253(T) (96.0 %). The major cellular fatty acids were C(16 : 0), C(18 : 1)/C(18 : 1)ω7c, C(17 : 0) cyclo and summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH). Based on the phylogenetic analysis, DNA-DNA hybridization, whole-cell fatty acid composition and biochemical characteristics, strain BF-3(T) was clearly distinct from type strains of other recognized species of the genus Comamonas and, as such, represents a novel species of the genus Comamonas, for which the name Comamonas zonglianii sp. nov. is proposed. The type strain is BF-3(T) (=CCTCC AB 209170(T) =DSM 22523(T)).