Roles of exopolysaccharides and lipopolysaccharides in the adsorption of the Siphovirus phage NM8 to Rhizobium meliloti M11S cells
Roles of exopolysaccharides and lipopolysaccharides in the adsorption of the Siphovirus phage NM8 to Rhizobium meliloti M11S cells
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DOI：
10.1007/s002849900130
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发表时间：
1996-12-01
影响因子：
2.6
通讯作者：
Hornez, JP
中科院分区：
文献类型：
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作者：
Defives, C;Werquin, M;Hornez, JP
根瘤菌M11S产生的胞外多糖包被非特异性地抑制噬菌体NM8的吸附。但脂多糖（LPS）具有特异性抑制作用。只有由葡萄糖、氨基葡萄糖、半乳糖、3-脱氧-d -甘露糖醛酸（KDO）和大量唾液酸组成的LPS多糖部分抑制噬菌体吸附；脂质A部分和细胞葡聚糖都没有参与。缺乏唾液酸的根瘤菌不能与噬菌体NM8结合。n -乙酰神经氨酸特异性凝集素对噬菌体结合的抑制表明，噬菌体NM8与唾液酸结合。单糖预孵育表明噬菌体的失活对n -乙酰神经氨酸具有很强的立体特异性。n -乙酰氨基葡萄糖对噬菌体的吸附也有强烈的抑制作用，而LPS中不存在乙酰氨基葡萄糖。因此，噬菌体NM8的受体似乎是一个糖类位点，可能涉及唾液酸的乙酰基。
The exopolysaccharides produced by Rhizobium meliloti M11S inhibited nonspecifically the adsorption of phage NM8 by coating the cells. But lipopolysaccharides (LPS) had a specific inhibitory effect. Only the polysaccharide moiety of LPS, composed of glucose, glucosamine, galactose, 3-deoxy-D-manno-octulosonic acid (KDO), and large amounts of sialic acid, inhibited phage adsorption; neither the lipid A moiety nor a cellular glucan was involved. Rhizobium strains lacking sialic acids did not bind phage NM8. Inhibition of phage binding by lectin specific for N-acetylneuraminic acid demonstrated that phage NM8 bound to sialic acids. Preincubation of the phage with monosaccharides showed that inactivation of phage was very stereospecific for N-acetylneuraminic acid. Phage adsorption was also strongly inhibited by N-acetylglucosamine, which is not present in the LPS. Therefore, the receptor for phage NM8 appears to be a saccharide site, probably involving the acetyl groups of sialic acids.