Reconfigurable Carbon Nanotube Multiplexed Sensing Devices.
Reconfigurable Carbon Nanotube Multiplexed Sensing Devices.
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DOI：
10.1021/acs.nanolett.8b00856
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发表时间：
2018-06
期刊：
影响因子：
10.8
通讯作者：
Xinzhao Xu;P. Clément;Johnas Eklöf-Österberg;N. Kelley-Loughnane;K. Moth‐Poulsen;J. Chávez;M. Palma
中科院分区：
文献类型：
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作者：
Xinzhao Xu;P. Clément;Johnas Eklöf-Österberg;N. Kelley-Loughnane;K. Moth‐Poulsen;J. Chávez;M. Palma
在此，我们报道了基于单壁碳纳米管（SWCNTs）的可重构和溶液可加工的具有多传感能力的纳米级生物传感器的制造。通过低成本的介质电泳（DEP）方法，将不同的dna包裹（因此是水溶性的）碳纳米管从溶液中固定到同一芯片上不同的预图案电极上。CNTs被特异的、不同的适体序列功能化，作为应激和神经创伤条件下生物标志物的选择性识别元件。成功地进行了三种不同生物标志物的多重检测，并在血清中实现了皮质醇、脱氢表雄酮硫酸酯（DHEAS）和神经肽Y （NPY）的实时检测，分别降至生理相关浓度为50 nM、10 nM和500 pM。此外，制备的纳米级器件通过简单的清洁程序被证明是可重构和可重复使用的。所提出的策略的普遍适用性，以及从水溶液中制造的简易设备，为开发下一代低功耗便携式诊断分析方法同时监测不同健康参数提供了巨大的潜力。
Here we report on the fabrication of reconfigurable and solution processable nanoscale biosensors with multisensing capability, based on single-walled carbon nanotubes (SWCNTs). Distinct DNA-wrapped (hence water-soluble) CNTs were immobilized from solution onto different prepatterned electrodes on the same chip, via a low-cost dielectrophoresis (DEP) methodology. The CNTs were functionalized with specific, and different, aptamer sequences that were employed as selective recognition elements for biomarkers indicative of stress and neuro-trauma conditions. Multiplexed detection of three different biomarkers was successfully performed, and real-time detection was achieved in serum down to physiologically relevant concentrations of 50 nM, 10 nM, and 500 pM for cortisol, dehydroepiandrosterone-sulfate (DHEAS), and neuropeptide Y (NPY), respectively. Additionally, the fabricated nanoscale devices were shown to be reconfigurable and reusable via a simple cleaning procedure. The general applicability of the strategy presented, and the facile device fabrication from aqueous solution, hold great potential for the development of the next generation of low power consumption portable diagnostic assays for the simultaneous monitoring of different health parameters.