Skeletal muscle cell proliferation and differentiation on polypyrrole substrates doped with extracellular matrix components
Skeletal muscle cell proliferation and differentiation on polypyrrole substrates doped with extracellular matrix components
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DOI：
10.1016/j.biomaterials.2009.06.059
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发表时间：
2009-10-01
期刊：
影响因子：
14
通讯作者：
Wallace, Gordon G.
中科院分区：
文献类型：
--
作者：
Gilmore, Kerry J.;Kita, Magdalena;Wallace, Gordon G.
导电聚合物已被开发作为一系列细胞类型的体外研究的基质，包括电兴奋细胞，如神经和平滑肌。本研究的目的是优化和改进一系列聚吡咯材料，以作为电刺激分化骨骼肌成肌细胞的底物。虽然所有的聚合物材料提供了合适的基板成肌细胞的粘附和增殖，显着的差异变得明显的低血清条件下用于分化的原代成肌细胞。这项工作的意义在于设计和控制聚合物材料，以促进骨骼肌细胞增殖和/或分化的不同阶段，为这种组织的工程化提供机会。因此，本文不仅是一个生物相容性评估，但合成条件如何影响细胞反应方面的最终结果的全面研究。(C)2009爱思唯尔有限公司版权所有。
Conducting polymers have been developed as substrates for in vitro studies with a range of cell types including electrically-excitable cells such as nerve and smooth muscle. The goal of this study was to optimise and characterise a range of polypyrrole materials to act as substrates for electrical stimulation of differentiating skeletal myoblasts. Although all of the polymer materials provided suitable substrates for myoblast adhesion and proliferation, significant differences became apparent under the low-serum conditions used for differentiation of primary myoblasts. The significance of the work lies in the design and control of polymer materials to facilitate different stages of skeletal muscle cell proliferation and/or differentiation, opening up opportunities for engineering of this tissue. This paper therefore constitutes not just a biocompatibility assessment but a comprehensive study of how synthesis conditions affect the final outcome in terms of cell response. (C) 2009 Elsevier Ltd. All rights reserved.