Neutrophils are associated with capillary closure in spontaneously diabetic monkey retinas
Neutrophils are associated with capillary closure in spontaneously diabetic monkey retinas
复制标题
DOI：
10.2337/diabetes.54.5.1534
复制
发表时间：
2005-05-01
期刊：
影响因子：
7.7
通讯作者：
Lutty, GA
中科院分区：
文献类型：
--
作者：
Kim, SY;Johnson, MA;Lutty, GA
2型糖尿病在肥胖衰老的恒河猴（Macaca mulatta）中自发发生。本研究探讨了多形核白细胞与视网膜病变发展之间的关系。测定了15只糖尿病猴和6只非糖尿病猴的血压和血糖水平。在动物最终下降和择期尸检开始前测定总胆固醇、LDL胆固醇、HDL胆固醇和甘油三酯的血浆水平。孵育视网膜以检测ADDRESS（标记存活的视网膜血管）和非特异性酯酶（标记中性粒细胞）活性。计算每平方毫米视网膜的多形性白细胞数。在将视网膜平坦包埋在乙二醇甲基丙烯酸酯中后，通过感兴趣的区域获取组织切片并在显微镜下观察。血管内多形核白细胞的数量增加，与视网膜毛细血管无灌注区相邻。糖尿病视网膜中每平方毫米的多形核白细胞数（6.91 ± 5.01）显著高于正常视网膜（1.45 ± 1.62，P = 0.018）。糖尿病患者高血压的严重程度也与多形核白细胞数量增加显著相关（P = 0.02）。每平方毫米的多形核白细胞数与总胆固醇（R = 0.907）、低密度脂蛋白胆固醇（R = 0.875）、总胆固醇与高密度脂蛋白胆固醇比率（R = 0.86）和总甘油三酯（R = 0.888）水平之间呈显著的指数正相关。这项研究表明，糖尿病的严重程度和视网膜病变的发展与糖尿病猴视网膜中多形核白细胞数量的增加有关。高血压、LDL胆固醇和甘油三酯的高血浆水平以及HDL胆固醇的低血浆水平也与视网膜中的多形核白细胞增加相关。
Type 2 diabetes develops spontaneously in obese aging rhesus monkeys (Macaca mulatta). This study investigates the association between polymorphonuclear lenkocytes and development of retinopathy. Blood pressure and plasma glucose levels were determined in 15 diabetic and 6 nondiabetic monkeys. The plasma levels of total cholesterol, LDL cholesterol, HDL cholesterol, and triglycerides were determined just before the start of the animal's final decline and elective necropsy. Retinas were incubated for ADPase (labels viable retinal blood vessels) and nonspecific esterase (labels neutrophils) activities. Polymorphonuclear leukocytes were counted per millimeter squared of retina. After the retina was flat-embedded in glycol methacrylate, tissue sections were taken through areas of interest and observed microscopically. Elevated numbers of intravascular polymorphonuclear leukocytes were present adjacent to areas with retinal capillary nonperfusion. There were significantly more polymorphonuclear leukocytes per millimeter squared in diabetic retinas (6.91 +/- 5.01) compared with normal retinas (1.45 +/- 1.62, P = 0.018). Severity of hypertension in diabetes was also significantly associated with greater numbers of polymorphonuclear leukocytes (P = 0.02). There was a significant positive exponential correlation between the number of polymorphonuclear leukocytes per millimeter squared and the level of total cholesterol (R = 0.907), LDL cholesterol (R = 0.875), the total cholesterol-to-HDL cholesterol ratio (R = 0.86), and total triglycerides (R = 0.888). This study demonstrates that severity of diabetes and the development of retinopathy are associated with increased numbers of polymorphonuclear leukocytes in the retina of diabetic monkeys. Hypertension, high plasma levels of LDL cholesterol and triglycerides, and low plasma levels of HDL cholesterol also are associated with increased polymorphonuclear leukocytes in retina.