Comparative analysis of mesenchymal stem cells from adult mouse adipose, muscle, and fetal muscle
Comparative analysis of mesenchymal stem cells from adult mouse adipose, muscle, and fetal muscle
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成年小鼠脂肪、肌肉和胎儿肌肉间充质干细胞的比较分析
DOI：
10.1007/s11033-012-2129-3
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发表时间：
2013-02-01
影响因子：
2.8
通讯作者：
Chen, Daiwen
中科院分区：
文献类型：
--
作者：
Lei, Hulong;Yu, Bing;Chen, Daiwen
近期，越来越多的证据支持成体干细胞是组织生长和修复的自然系统的一部分。本研究聚焦于成年脂肪来源的间充质干细胞（ADSCs）、骨骼肌来源的间充质干细胞（MDSCs）和胎儿肌肉来源的间充质干细胞（FMSCs）在生物学特性方面的差异，这是细胞治疗成功的关键。第3代MDSCs和FMSCs的干细胞抗原1（Sca - 1）表达量是第1代的两倍（P < 0.0001）。经过28天的肌源性诱导后，MDSCs中骨骼肌特异性基因的表达水平高于FMSCs（P < 0.01），并且在这三种细胞中ADSCs的表达水平最低（P < 0.01）。此外，通过免疫荧光或实时定量PCR未检测到ADSCs中M - Cad和MyHC的表达。再者，经过14天的成脂诱导后，ADSCs中PPARγ2、脂蛋白脂肪酶（LPL）和脂肪酸结合蛋白2（aP2）的mRNA表达高于MDSCs（P < 0.01）。此外，经过28天的成骨诱导后，成年或胎儿肌肉来源的间充质干细胞表达的骨钙素（OCN）和骨桥蛋白（OPN）高于ADSCs（P < 0.01）。综上所述，我们的研究结果表明细胞来源和发育阶段对间充质干细胞的生物学特性有重大影响，有必要对所有这些问题进行适当考虑。
Recently, increasing evidence supports that adult stem cells are the part of a natural system for tissue growth and repair. This study focused on the differences of mesenchymal stem cells from adult adipose (ADSCs), skeletal muscle (MDSCs) and fetal muscle (FMSCs) in biological characteristics, which is the key to cell therapy success. Stem cell antigen 1 (Sca-1) expression of MDSCs and FMSCs at passage 3 was two times more than that at passage 1 (P< 0.0001). After 28-day myogenic induction, higher expression levels of skeletal muscle-specific genes were observed in MDSCs than FMSCs (P< 0.01), and the lowest expression levels were demonstrated in ADSCs among three cells (P< 0.01). Besides, M-Cad and MyHC expressions in ADSCs were not detected by immunofluorescence or real-time quantitative PCR. Furthermore, after 14 days adipogenic induction, PPARγ2, LPL and aP2 mRNA expressions were higher in ADSCs vs. MDSCs (P< 0.01). Besides, MSCs from adult or fetal muscle expressed higher OCN and OPN than ADSCs after 28 days osteogenic induction (P< 0.01). Taken together, our results suggested that cell source and developmental stage had great impacts on biological properties of mesenchymal stem cells, and proper consideration of all the issues is necessary.