Establishment of a cell line with phenotypes of chondrocyte from a human osteogenic sarcoma of the mandible.
Establishment of a cell line with phenotypes of chondrocyte from a human osteogenic sarcoma of the mandible.
复制标题
建立具有来自人下颌骨成骨肉瘤的软骨细胞表型的细胞系。
DOI：
10.1034/j.1600-0714.2000.290706.x
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发表时间：
2000
影响因子：
3.3
通讯作者：
M. Nagumo
中科院分区：
文献类型：
--
作者：
K. Yagami;S. Kakuta;H. Tachibana;Y. Kimura;M. Nagumo
我们已成功将人类下颌骨成骨肉瘤移植到无胸腺小鼠体内。移植的肿瘤显示出明显的软骨形成和矿化。最近，从移植肿瘤中建立了具有软骨细胞表型的细胞系（USAC）。 USAC 细胞在稀疏培养中呈星状或纺锤形，但在亚汇合至汇合时呈多边形或球形。长期培养，细胞凝集，形成钙化结节。通过免疫组织化学染色和蛋白质印迹分析检测 I、II 和 X 型胶原蛋白的产生。 I 型胶原蛋白在星状或纺锤状细胞中强烈表达。尽管 II 型胶原蛋白在培养过程中通常存在于所有细胞中，但在汇合时，它在多边形细胞中被强烈染色。 X 型胶原蛋白可见于钙化结节周围的大多边形细胞中。在汇合阶段和结节中观察到明显的[35S]-硫酸盐摄取和异染。结节周围细胞碱性磷酸酶阳性，结节中心被茜素红染色。使用无胸腺小鼠的扩散室进行的体内实验证实了软骨形成的潜力。这些观察结果表明 USAC 细胞保持了软骨细胞祖细胞的特征，因此可以作为研究软骨形成的连续事件和病态软骨内钙化过程的有用模型。该实验还证明，将肿瘤组织移植到无胸腺小鼠体内是建立细胞系的便捷策略。
We have succeeded in transplanting a human osteogenic sarcoma of the mandible into athymic mice. The transplanted tumor showed marked chondrogenesis and mineralization. Recently, a cell line (USAC) with phenotypes of chondrocyte has been established from the transplanted tumor. USAC cells were stellate or spindle-shaped in sparse culture, but polygonal or spherical at sub-confluency to confluency. In long-term culture, the cells were condensed and calcified nodules were formed. Production of types I, II and X collagen were detected by immunohistochemical staining and Western blot analysis. Type I collagen was strongly expressed in the stellate or spindle-shaped cells. Although type II collagen was usually present in all cells during culture, it was strongly stained in polygonal cells at confluency. Type X collagen was seen in large polygonal cells around calcified nodules. Marked [35S]-sulfate uptake and metachromasia were seen at the confluent stage and in the nodule. The cells around the nodules were positive for alkaline phosphatase, and the center of the nodules was stained with alizarin red. The potentiality of cartilage formation was confirmed by in vivo experiments using a diffusion chamber in athymic mice. These observations indicate that USAC cells maintain characteristics of chondrocyte progenitor cells and thus may serve as a useful model to study the sequential events of chondrogenesis and the process of morbid endochondral calcification. This experiment also demonstrated that transplantation of tumor tissue into athymic mice is a convenient strategy for establishment of a cell line.