Antiviral activity of an oligo(nucleoside methylphosphonate) that targets HSV-1 immediate-early pre-mRNA 4,5 is augmented by cotreatment with replication-defective adenovirus.
Antiviral activity of an oligo(nucleoside methylphosphonate) that targets HSV-1 immediate-early pre-mRNA 4,5 is augmented by cotreatment with replication-defective adenovirus.
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与复制缺陷型腺病毒共同治疗可增强针对 HSV-1 立即早期前 mRNA 4,5 的寡聚（核苷甲基膦酸酯）的抗病毒活性。
DOI：
10.1089/ard.1995.5.243
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
Aurelian,L
中科院分区：
文献类型：
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作者：
Kulka,M;Aurelian,L
Replication-defective adenovirusp259Acaused a 400-fold increase in the sequence-specific antiherpetic activity of oligo(nucleoside methylphosphonate) (ONMP) IE4,5SA. Herpes simplex virus type 1 (HSV-1) growth was not inhibited in cells exposed top259Ain the absence of IE4,5SA or in cells cotreated with I E4,5SA and heated (10 minutes, 90°C)p259Avirus. Fluorescent microscopy of Vero cells treated with BODIPY-conjugated IE4,5SA revealed intracellular localization within endocytic-like vesicles with minimal cytoplasmic and intranuclear distribution. Diffuse staining over the entire cell was observed in cell cotreated with the BODIPY-conjugated IE4,5SA andp259Avirus. This effect was not observed in cells cotreated with the BODIPY-conjugated ONMP and heatedp259Avirus. We interpret these findings to indicate thatp259Aaugments IE4,5SA antiherpetic activity presumably via its ability to increase ONMP uptake and release from endocytic-like vesicles.
