Intraclonal genome diversity of the major Pseudomonas aeruginosa clones C and PA14
Intraclonal genome diversity of the major Pseudomonas aeruginosa clones C and PA14
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DOI：
10.1111/1758-2229.12372
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发表时间：
2016-04-01
影响因子：
3.3
通讯作者：
Tuemmler, Burkhard
中科院分区：
文献类型：
--
作者：
Fischer, Sebastian;Klockgether, Jens;Tuemmler, Burkhard
细菌种群在亚种水平上分化为克隆复合体。对采自无生命环境、急性和慢性感染的两个优势克隆C和PA14的100株铜绿假单胞菌的克隆内基因组多样性进行了研究。核心基因组在克隆成员间高度保守，克隆内中位数为8x10(-6)的克隆C和2x10(-5)的克隆PA14。另一方面，辅助基因组的组成在克隆内和在不相关克隆之间一样可变。每一种菌株都携带大量独特的基因。这两个主要分布在世界各地的铜绿假单胞菌克隆将一个几乎不变的核心与通过水平转移传播的遗传元素的灵活获得和损失结合在一起。
Bacterial populations differentiate at the subspecies level into clonal complexes. Intraclonal genome diversity was studied in 100 isolates of the two dominant Pseudomonas aeruginosa clones C and PA14 collected from the inanimate environment, acute and chronic infections. The core genome was highly conserved among clone members with a median pairwise within-clone single nucleotide sequence diversity of 8x10(-6) for clone C and 2x10(-5) for clone PA14. The composition of the accessory genome was, on the other hand, as variable within the clone as between unrelated clones. Each strain carried a large cargo of unique genes. The two dominant worldwide distributed P. aeruginosa clones combine an almost invariant core with the flexible gain and loss of genetic elements that spread by horizontal transfer.