Tumor suppressor gene BRCA-1 is expressed by embryonic and adult neural stem cells and involved in cell proliferation
Tumor suppressor gene BRCA-1 is expressed by embryonic and adult neural stem cells and involved in cell proliferation
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DOI：
10.1002/jnr.10546
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发表时间：
2003-03-15
影响因子：
4.2
通讯作者：
Lindholm, D
中科院分区：
文献类型：
--
作者：
Korhonen, L;Brännvall, K;Lindholm, D
BRCA-1是一种肿瘤抑制基因，在DNA修复和细胞生长控制中发挥作用。在这里，我们表明，BRCA-1 mRNA表达的胚胎大鼠脑，并定位于神经上皮细胞含有神经元前体细胞。BRCA-1的表达在大鼠脑发育过程中减少，但BRCA-1在出生后通过发育中小脑中增殖的神经元前体细胞表达。神经干细胞（NSC）从胚胎大鼠脑和培养在表皮生长因子的存在下，BRCA-1呈阳性。如RNA和蛋白质分析所示，诱导NSC分化导致BRCA-1表达下调。除胚胎细胞外，BRCA-1还存在于从成年大鼠脑制备的NSC中。在成年大鼠中，BRCA 1在脑室壁和脉络丛中的细胞中表达。结果表明，BRCA-1存在于胚胎和成年大鼠NSC中，并且表达与NSC增殖有关。(C)2003 Wiley-Liss，Inc.
BRCA-1 is a tumor suppressor gene that plays a role in DNA repair and cellular growth control. Here we show that BRCA-1 mRNA is expressed by embryonic rat brain and is localized to the neuroepithelium containing neuronal precursor cells. The expression of BRCA-1 decreases during rat brain development, but BRCA-1 is expressed postnatally by proliferating neuronal precursor cells in the developing cerebellum. Neural stem cells (NSC) prepared from embryonic rat brain and cultured in the presence of epidermal growth factor were positive for BRCA-1. Induction of NSC differentiation resulted in down-regulation of BRCA-1 expression as shown by RNA and protein analyses. In addition to embryonic cells, BRCA-1 is also present in NSC prepared from adult rat brain. In adult rats, BRCA1 was expressed by cells in the walls of brain ventricles and in choroid plexus. The results show that BRCA-1 is present in embryonic and adult rat NSC and that the expression is linked to NSC proliferation. (C) 2003 Wiley-Liss, Inc.