Telomere length regulation during postnatal development and ageing in Mus spretus
Telomere length regulation during postnatal development and ageing in Mus spretus
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DOI：
10.1093/nar/25.15.3051
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发表时间：
1997-08-01
影响因子：
14.9
通讯作者：
Prowse, KR
中科院分区：
文献类型：
--
作者：
CovielloMcLaughlin, GM;Prowse, KR
端粒缩短与人类的复制性衰老有因果关系。为了检查小鼠中端粒长度与衰老之间的关系，我们利用小鼠作为模型物种，因为它具有与人类大致相同长度的端粒长度。从>180只不同年龄的小鼠的肝脏、肾脏、脾脏、大脑和睾丸分析端粒长度和端粒酶，虽然各组织的端粒长度不均匀，但在脾脏和脑中发现了显著的端粒长度变化，而在肝脏、睾丸和肾脏中没有发现端粒长度变化。在来自M.spretus和M.spretus x C57 BL/6 F1小鼠的组织中发现了平均末端限制性片段长度的性别差异，其中可以测量M. spretus大小的端粒涂片。对单个M.spretus内的组织端粒长度的排序的比较显示，某些组织倾向于比其他组织更长，并且这种排序也延伸到M.spretus x C57 BL/6 F1小鼠的组织。这些数据表明，单个组织内的端粒长度是独立调节的，并且是遗传控制的。
Telomere shortening has been causally implicated in replicative senescence in humans, To examine the relationship between telomere length and ageing in mice, we have utilized Mus spretus as a model species because it has telomere lengths of approximately the same length as humans, Telomere length and telomerase were analyzed from liver, kidney, spleen, brain and testis from >180 M.spretus male and female mice of different ages, Although telomere lengths for each tissue were heterogeneous, significant changes in telomere lengths were found in spleen and brain, but not in liver, testis or kidney, Telomerase activity was abundant in liver and testis, but weak to non-detectable in spleen, kidney and brain. Gender differences in mean terminal restriction fragment length were discovered in tissues from M.spretus and from M.spretus x C57BL/6 F1 mice, in which a M.spretus-sized telomeric smear could be measured. The comparison of the rank order of tissue telomere lengths within individual M.spretus showed that certain tissues tended to be longer than the others, and this ranking also extended to tissues of the M.spretus x C57BL/6 F1 mice, These data suggest that telomere lengths within individual tissues are regulated independently and are genetically controlled.