Label-Free Imaging of Dynamic and Transient Calcium Signaling in Single Cells
Label-Free Imaging of Dynamic and Transient Calcium Signaling in Single Cells
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单细胞动态和瞬时钙信号传导的无标记成像
DOI：
10.1002/anie.201505991
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发表时间：
2015-11-09
影响因子：
16.6
通讯作者：
Li, Jinghong
中科院分区：
文献类型：
--
作者：
Lu, Jin;Li, Jinghong
细胞信号传导包括发生在不同时间和空间尺度上的各种事件，从毫秒到小时，从单个生物分子到细胞群体。通路的复杂性需要新技术的发展，以检测整体的信号活动，而不是局限于量化一个单一的事件。一种基于等离子体的电化学阻抗显微镜（P-EIM）可以提供这些数据，具有优异的时间和空间分辨率，并且不需要添加任何标记进行检测，现在已经开发出来。因此，研究了g蛋白偶联受体（GPCR）刺激早期的高动态和瞬时钙信号活动。结果表明，一部分细胞对激动剂的刺激更敏感，并研究了单个HeLa细胞在激动剂激活钙通量过程中局部分布的异质性和离子通道的瞬时活性。
Cell signaling consists of diverse events that occur at various temporal and spatial scales, ranging from milliseconds to hours and from single biomolecules to cell populations. The pathway complexities require the development of new techniques that detect the overall signaling activities and are not limited to quantifying a single event. A plasmonic-based electrochemical impedance microscope (P-EIM) that can provide such data with excellent temporal and spatial resolution and does not require the addition of any labels for detection has now been developed. The highly dynamic and transient calcium signaling activities at the early stage of G-protein-coupled receptor (GPCR) stimulation were thus studied. It could be shown that a subpopulation of cells is more responsive towards agonist stimulation, and the heterogeneity of the local distributions and the transient activities of the ion channels during agonist-activated calcium flux in single HeLa cells were investigated.