Expression of intercellular lipid transport and cholesterol metabolism genes in eggs and early larvae stages of turbot, Scophthalmus maximus, a marine aquaculture species
Expression of intercellular lipid transport and cholesterol metabolism genes in eggs and early larvae stages of turbot, Scophthalmus maximus, a marine aquaculture species
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DOI：
10.1007/s00227-015-2706-9
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发表时间：
2015-08-01
期刊：
影响因子：
2.4
通讯作者：
Castro, L. F. C.
中科院分区：
文献类型：
--
作者：
Cunha, I.;Galante-Oliveira, S.;Castro, L. F. C.
生长和能量转移严重依赖于脂质分子在组织和细胞区室之间的有效运输。这一过程在卵和eleutheroembryos中是特异性的，当位于卵黄囊的能量和结构脂质需要被动员以并入新形成的胚胎或产生能量时。在这里，我们描述了11个基因的转录谱，编码的蛋白质参与细胞间的脂质运输和胆固醇代谢的海洋硬骨鱼（大菱鲆）的早期发展，从脊索形成期后开口。描述了基因（apoA 1、apoB 100、apoE、cetp、mtp、pltp、lipC、lpl、hmgcr 1、soat 1、lcat）的mRNA表达模式，并将其与先前公开的幼虫中的脂质水平和来自相同实验的PPARS-过氧化物酶体增殖物激活受体-mRNA水平相关（库尼亚等人，Mar Genomics 10：17-25，2013）。我们的研究结果表明，转录的基因负责载脂蛋白的生产开始前不久孵化和活动下降沿着的发展。相反，负责胆固醇合成的基因在发育早期转录水平较低，随后其活性增加。载脂蛋白和其他与胆固醇逆向转运相关的基因可能受Ppar α 2的控制，而参与胆固醇合成的细胞外脂质转运蛋白和酶的表达可能同时受Ppar α 1和Ppar γ的控制。一般来说，观察到的参与脂质转运的基因的转录与幼虫的脂质组成和脂质代谢主要调节因子（例如核受体-PPARs）的转录一致。
Growth and energy transfer are critically dependent on effective transport of lipid molecules between tissues and cellular compartments. This process is specific in egg and eleutheroembryos, when energetic and structural lipids, located at the yolk sac, need to be mobilized in order to be incorporated in the new forming embryo, or to produce energy. Here, we describe the transcriptional profile of 11 genes that codify for proteins involved in intercellular lipid transport and cholesterol metabolism during the early development of a marine teleost fish (Scophthalmus maximus), from notochord formation to the period beyond mouth opening. The mRNA expression pattern of genes (apoA1, apoB100, apoE, cetp, mtp, pltp, lipC, lpl, hmgcr1, soat1, lcat) is described and related to previously published lipid levels in larvae and PPARs-peroxisome proliferator-activated receptors-mRNA levels from the same experiment (Cunha et al. in Mar Genomics 10:17-25, 2013). Our findings show that the transcription of genes responsible for apolipoproteins production starts soon before hatching and that activities decline along the development. In contrast, genes responsible for cholesterol synthesis have a low transcription level early in the development and their activity increases later. Apolipoproteins and other genes related to reverse cholesterol transport are possibly under the control of Ppar alpha 2, while the expression of extracellular lipid transfer proteins and enzymes involved in cholesterol synthesis is possibly under the simultaneous control of Ppar alpha 1 and Ppar gamma. Generally, the observed transcription of genes involved in lipid transport is in accordance with the lipid composition of the larvae and transcription of master regulators of lipid metabolism such as the nuclear receptors-PPARs.