Inhibition of tomato fruit ripening by 1-MCP, wortmannin and hexanal is associated with a decrease in transcript levels of phospholipase D and other ripening related genes
Inhibition of tomato fruit ripening by 1-MCP, wortmannin and hexanal is associated with a decrease in transcript levels of phospholipase D and other ripening related genes
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DOI：
10.1016/j.postharvbio.2018.02.009
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发表时间：
2018-06-01
影响因子：
7
通讯作者：
Paliyath, Gopinadhan
中科院分区：
文献类型：
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作者：
Dek, Mohd Sabri Pak;Padmanabhan, Priya;Paliyath, Gopinadhan
果膜变质是贮藏过程中果实衰老进程和品质损失的内在表现。用于延长保质期和质量的采后技术旨在通过下调或阻断乙烯作用来减少膜损伤。本研究用乙烯受体（ETR）、磷脂酰肌醇3-激酶（PI3K）和磷脂酶D （PLD）抑制剂处理成熟的绿番茄果实，这些抑制剂都被认为是调节果实成熟的靶点。使用的抑制剂包括1-甲基环丙烯（1-MCP，乙烯受体阻滞剂），wortmannin （PI3K抑制剂）和己醛（PLD抑制剂）。水果以最佳水平的抑制剂处理，并在21℃下储存10天。与1-MCP处理的番茄一样，wortmannin处理的番茄颜色发育明显延迟；而呼吸、硬度和乙烯演化的变化与对照果非常相似。己醛延缓了这些变化的发生；而1-MCP和wortmannin则阻断了成熟过程。参与乙烯信号传导、磷酸肌肽代谢和番茄红素合成的关键基因表达水平的变化表明，PI3K和PLD在乙烯信号传导中可能发挥作用。此外，用这三种抑制剂处理的果实显示PLD转录水平显著降低；表明，调控PLD基因表达是调控成熟的共同关键调控点。PLD水平降低可能会减少膜脂分解代谢和磷脂酸（PA）的产生，磷脂酸是通过下游组分调节乙烯信号的中间物。
Membrane deterioration is an inherent aspect of the advancement in senescence and loss in fruit quality during storage. Postharvest technologies used for extending shelf life and quality are targeted to reduce membrane damage through downregulating or blocking ethylene action. In this study, mature green tomato fruit were treated with inhibitors of ethylene receptor (ETR), phosphatidylinositol 3-kinase (PI3K) and phospholipase D (PLD), all recognized to be targets of regulation of fruit ripening. The inhibitors used included 1-methylcyclopropene (1-MCP, an ethylene receptor blocker), wortmannin (an inhibitor of PI3K), and hexanal (a PLD inhibitor). Fruit were treated at optimal levels of the inhibitors and were stored at 21 degrees C for 10 days. Color development was strongly delayed in wortmannin treated tomatoes just as in 1-MCP treated fruit; while, changes in respiration, firmness and ethylene evolution were very similar to that of control fruit. Hexanal delayed the initiation of these changes; while 1-MCP and wortmannin blocked the ripening process. Changes in expression levels of key genes involved in ethylene signalling, phosphoinositide metabolism, and lycopene synthesis that occurred in response to inhibitors, suggested potential roles for PI3K and PLD in ethylene signalling. Furthermore, fruit treated with all the three inhibitors showed a marked reduction in PLD transcript levels; suggesting that, regulation of PLD gene expression is a common critical regulatory point that regulates ripening. Lowered PLD levels may reduce membrane lipid catabolism and the generation of phosphatidic acid (PA), an intermediate in ethylene signalling regulation through downstream components.