Effects of 50 Hz magnetic field exposure on the rate of DNA synthesis by normal human fibroblasts.
Effects of 50 Hz magnetic field exposure on the rate of DNA synthesis by normal human fibroblasts.
复制标题
50 Hz 磁场暴露对正常人成纤维细胞 DNA 合成速率的影响。
DOI：
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发表时间：
1996
影响因子：
2.6
通讯作者：
R. Saunders
中科院分区：
文献类型：
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作者：
N. Cridland;T. Cragg;R. Haylock;R. Saunders
对暴露于工频磁场的潜在不利生物效应的兴趣集中在这些磁场可能通过增加细胞增殖速度影响肿瘤促进的可能性上。为了研究暴露在磁场中是否真的会影响细胞增殖的速度，正常的人类成纤维细胞被血清饥饿过夜，然后在一个专门建造的设施中暴露在50赫兹的磁场中。DNA合成速率是衡量细胞增殖的指标，并通过将[3H]-胸腺嘧啶掺入大分子物质来测定。将暴露细胞的DNA合成率与置于标准CO2培养箱中的对照培养物进行比较，在标准CO2培养箱中，细胞暴露于< 200 nT的背景磁场中。阳性对照保持在相同的CO2培养箱中，但用人重组成纤维细胞生长因子处理，以检查细胞对生长刺激的反应。50 Hz的磁场和20微t至20毫特之间的磁通密度范围对暴露30小时的细胞的DNA合成速率没有可检测到的影响。
Interest in the potential adverse biological effects of exposure to power-frequency magnetic fields has centred on the possibility that these fields may influence tumour promotion, possibly by increasing the rate of cell proliferation. In order to investigate whether exposure to magnetic fields can indeed affect the rate of cell proliferation, normal human fibroblasts were serum starved overnight and then exposed to 50 Hz magnetic fields in a purpose-built facility. The rate of DNA synthesis was taken as a measure of cell proliferation, and was determined by following the incorporation of [3H]-thymidine into macromolecular material. The rate of DNA synthesis in exposed cells was compared with that in control cultures maintained in a standard CO2 incubator where they were exposed to background magnetic fields of < 200 nT. Positive controls were maintained in the same CO2 incubator, but were treated with human recombinant fibroblast growth factor to check that the cells were responsive to growth stimuli. Magnetic fields at 50 Hz and at a range of flux densities between 20 microT and 20 mT had no detectable effect on the rate of DNA synthesis by cells exposed for up to 30 h.