Genetic mapping of quantitative trait loci conditioning salt tolerance in wild soybean (Glycine soja) PI 483463
Genetic mapping of quantitative trait loci conditioning salt tolerance in wild soybean (Glycine soja) PI 483463
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DOI：
10.1007/s10681-013-0944-9
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发表时间：
2013-09-01
期刊：
影响因子：
1.9
通讯作者：
Lee, Jeong-Dong
中科院分区：
文献类型：
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作者：
Ha, Bo-Keun;Vuong, Tri D.;Lee, Jeong-Dong
大豆的耐盐性[甘氨酸max (L.)]稳定。]是由主要数量性状位点（QTL）或单个基因控制的。据报道，在大豆种质中，野生大豆引种PI 483463具有一个耐盐显性基因。本研究的目的是对PI 483463与Hutcheson杂交的重组自交系（RIL）群体的QTL进行遗传定位。采用简单序列重复（SSR）标记和通用大豆单核苷酸多态性（SNP）标记板（USLP 1.0）进行分子分型。采用1-5分制目视评定法，在温室内进行了两项独立试验。结果表明，pi483463的耐盐QTL定位在Satt255和BARC-038333-10036之间的3号染色体上。从PI 483463遗传的有利等位基因具有耐盐性，并对减少叶片烧焦具有加性效应。从同一杂交的F-3家族中获得66个同等位系，用于QTL的遗传确证。重组事件和盐反应数据的整合表明，该QTL位于第3号染色体上40,505,992核苷酸处的SSR03_1335和41,164,735核苷酸处的SSR03_1359之间约658 kb的区域。这一狭窄区域为进一步开展大豆耐盐基因克隆等基因组研究提供了有利条件。
Salt tolerance in soybean [Glycine max (L.) Merr.] is controlled by major quantitative trait loci (QTL) or single gene(s). Among soybean germplasm, wild soybean plant introduction PI 483463 was reported to have a single dominant gene for salt tolerance. The objective of this study was to genetically map the QTL in a recombinant inbred line (RIL) population derived from a cross between PI 483463 and Hutcheson. Simple sequence repeat (SSR) markers and universal soybean single nucleotide polymorphism (SNP) panel (the USLP 1.0) were utilized for molecular genotyping. The RILs were phenotyped in two independent tests in a greenhouse using a 1-5 scale visual rating method. The results showed that the salt tolerant QTL in PI 483463 was mapped to chromosome 3 in a genomic region between the Satt255 and BARC-038333-10036 markers. The favorable allele inherited from PI 483463 conferred tolerance to salinity and had an additive effect on reducing leaf scorch. A subset of 66 iso-lines was developed from the F-3 families of the same cross and was used for genetic confirmation of the QTL. The integration of recombination events and the salt reaction data indicate that the QTL is located in the region of approximately a 658 kb segment between SSR03_1335 at nucleotide 40,505,992 and SSR03_1359 at nucleotide 41,164,735 on chromosome 3. This narrow region can facilitate further genomic research for salt tolerance in soybean including cloning salt tolerance genes.