Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing
Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing
复制标题
用于超灵敏多重 DNA 生物传感的无核酸酶靶标回收信号放大
DOI：
10.1016/j.bios.2017.03.051
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发表时间：
2017
影响因子：
12.6
通讯作者：
Song Shiping
中科院分区：
文献类型：
--
作者：
Zhao Zhihan;Chen Shixing;Wang Jianbang;Su Jing;Xu Jiaqiang;Mathur Sanjay;Fan Chunhai;Song Shiping
无需基因扩增的超灵敏生物传感技术为直接检测DNA带来了巨大的希望。本文报道了一种结合靶循环信号放大策略和自制电化学装置的新型生物传感方法。特别地，通过链置换过程实现靶循环，而不需要任何核酸酶的帮助。在靶DNA的存在下，循环系统可以被激活以产生具有三个发夹DNA片段的级联组装步骤。每个循环过程都伴随着一个拆卸步骤，即最后一个发夹DNA片段在每次循环结束时从复合物中取代靶DNA，释放靶点以激活更多三叶形DNA结构的自组装。该方法能够在aM水平上检测目标DNA，并能区分目标DNA和干扰DNA，具有较高的灵敏度和选择性。重要的是，生物传感方法可以很好地与血清样品。
Ultrasensitive biosensing technologies without gene amplification held great promise for direct detection of DNA. Herein we report a novel biosensing method, combining target recycling signal-amplification strategy and a homemade electrochemical device. Especially, the target recycling was achieved by a strand displacement process, no needing the help of any nucleases. In the presence of target DNA, the recycling system could be activated to generate a cascade of assembly steps with three hairpin DNA segments. Each recycling process were accompanied by a disassembly step that the last hairpin DNA segment displaces target DNA from the complex at the end of each circulation, freeing targets to activate the self-assembly of more trefoil DNA structures. This biosensing method could detect target DNA at aM level and can distinguish target DNA from interfering DNAs, demonstrating its high sensitivity and high selectivity. Importantly, the biosensing method could work well with serum samples.