Role of antioxidants in prevention of pyrimidine dimer formation in UVB irradiated human HaCaT keratinocytes
Role of antioxidants in prevention of pyrimidine dimer formation in UVB irradiated human HaCaT keratinocytes
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DOI：
10.1016/j.biopha.2006.04.008
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发表时间：
2006-06-01
影响因子：
7.5
通讯作者：
Enk, Claes D.
中科院分区：
文献类型：
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作者：
Hochberg, Malka;Kohen, Ron;Enk, Claes D.
本研究旨在探讨中波紫外线(UVB)诱导人角质形成细胞环丁烷嘧啶二聚体(CPD)形成过程中活性氧簇(ROS)的作用，以及低分子抗氧化剂对CPD形成的调节作用。为了证明ROS的参与，我们研究了α-生育酚、坦帕明和二乙基二硫代氨基甲酸酯(DDC)对完整细胞和裸露DNA中CPD形成的保护能力。在抗氧化剂存在的情况下，用UVB照射HaCaT细胞和裸露DNA，用ELISA法测定CPD。我们发现这三种抗氧化剂都对UVB诱导的CPD的形成有保护作用。这种保护只在完整的细胞中观察到，而不是在裸DNA中观察到。由于一些被测试的抗氧化剂不具有紫外线吸收能力，我们的发现表明，在细胞环境中，ROS在CPD的形成中发挥了作用。(C)2006年爱思唯尔公司。版权所有。
The objective of the present study was to study the role of reactive oxygen species (ROS) in UVB induced cyclobutane pyrimidine dimer (CPD) formation in human keratinocytes, and to examine the modulating activity of low molecular weight antioxidants. To demonstrate the involvement of ROS, we examined the protective capacity of alpha-tocopherol, tempamine, and diethyldithiocarbamate (DDC) on CPD formation in intact cells and naked DNA. HaCaT cells and naked DNA in water solution were irradiated with UVB in the presence of the antioxidants and CPD was determined by ELISA. We found that all three antioxidants provided protection against UVB induced CPD formation. The protection was observed in intact cells only and not in naked DNA. Since some of the tested antioxidants do not possess UV absorbing qualities, our findings suggest that in a cellular environment ROS play a role in CPD formation. (c) 2006 Elsevier SAS. All rights reserved.