A method for the efficient cellular uptake and retention of small modified gold nanoparticles for the radiosensitization of cells
A method for the efficient cellular uptake and retention of small modified gold nanoparticles for the radiosensitization of cells
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DOI：
10.1016/j.nano.2014.03.011
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发表时间：
2014-08-01
影响因子：
5.4
通讯作者：
Veldwijk, Marlon R.
中科院分区：
文献类型：
--
作者：
Burger, Nina;Biswas, Abin;Veldwijk, Marlon R.
金纳米颗粒（GNP）增强光子的吸收，从而增加在nm-μ m范围内的俄歇电子/光电子的发射。然而，一个主要的缺点是它们的直径依赖性细胞摄取，最佳值类似于50 nm，这可能不会提供最佳的放射增敏作用。开发了一种方法来提高小国民生产总值的吸收。将GNP（10 nm）与DNA连接并通过瞬时转染（GNP-DT）转移到HeLa细胞中。用GNP-DT处理细胞导致强烈的核周局灶性积聚，而这对于GNP-T（缺乏DNA）是较暗和稀疏的，并且接近于GNP处理的细胞中的背景水平。使用临床相关的兆伏X射线，只有GNP-DT显示出对克隆存活的显著放射增敏作用（p = 0.005）。与未修饰的GNP相比，我们的新方法显著增加了摄取/保留，并改变了小GNP在细胞中的定位。这项工作最终能够研究不同大小的GNP的放射增敏作用。
Gold nanoparticles (GNP) enhance the absorbance of photons thereby increasing emission of Auger-/photoelectrons in the nm-mu m range. Yet, a major disadvantage is their diameter-dependent cellular uptake with an optimum of similar to 50 nm which may not offer optimal radiosensitization. A method was developed to enhance the uptake of small GNP. GNP (10 nm) were linked to DNA and transferred into HeLa cells by transient transfection (GNP-DT). Treatment of cells with GNP-DT resulted in a strong perinuclear focal accumulation, whereas this was dimmer and sparser for GNP-T (lacking DNA) and close to background levels in GNP-treated cells. Only GNP-DT showed a significant radiosensitizing effect (p = 0.005) on clonogenic survival using clinically relevant megavolt x-rays. Our novel method markedly increases the uptake/retention and alters the localization of small GNP in cells compared to unmodified GNP. This work finally enables studying the radiosensitizing effects of differentially sized GNP.