Developmental differences in the contractile response of isolated ovine tracheal smooth muscle cells.
Developmental differences in the contractile response of isolated ovine tracheal smooth muscle cells.
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DOI：
10.1002/ppul.21042
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发表时间：
2009-06
影响因子：
3.1
通讯作者：
Driska, Steven P.
中科院分区：
文献类型：
--
作者：
Laudadio, Rachel E.;Wolfson, Marla R.;Shaffer, Thomas H.;Driska, Steven P.
关键词：
先前的研究已经显示了气道平滑肌张力的发育差异。气道力学的差异可能基于不同年龄动物之间的细胞差异。我们开发了一种分离绵羊气管平滑肌细胞和测量缩短速度的方法[JAP 86（1）：427-35，1999]。本实验应用该技术研究了发育过程中气道平滑肌细胞收缩反应的变化。在这里，我们报告了早产儿（怀孕后110-124或125-140天），新生儿（出生后3-7天）和成人（9-36个月）细胞之间的差异。比较这些细胞的形态学、缩短速度和缩短百分比。新生细胞比成体细胞短而窄。成体细胞的最大缩短速度（45.1 μm/s）比新生细胞的最大缩短速度（11.1 ~ 25.1 μm/s）快。当将速度归一化为细胞长度时，成体和早产儿细胞之间没有差异，但新生儿（0.30 sec-1）和成体（0.54 sec-1）细胞之间存在显著差异。缩短率随年龄增长无显著性差异。在新生儿组内，形态测量、缩短或速度无显著差异。为了便于比较具有不同大小细胞的不同大小的ASM组织，我们还表示了相对于假设的1 mm组织段的缩短百分比和速度。以这种方式表示，所有年龄组的缩短量是相同的，但假设早产组织（125-140天）的预测最大速度显著大于新生儿。
Previous studies have shown developmental differences in smooth muscle tone of the airways. Differences in airway mechanics may be based upon cellular differences between animals of different ages. We developed a method [JAP 86(1): 427–35, 1999] for isolating ovine tracheal smooth muscle cells and for measuring shortening velocity. This technique was used to study the change in contractile response of the airway smooth muscle cell during development. Here we report differences between preterm (110–124 or 125–140 days post-conception), newborn (3–7 days postnatal) and adult (9–36 months) cells. These cells were compared with respect to morphometry, shortening velocity, and percent shortening. The neonatal cells were shorter and narrower than the adult cells. Maximum shortening velocity was faster for adult (45.1 μm/sec) than for neonatal cells (range 11.1 to 25.1 μm/s). When velocity was normalized to the cell length, there was no difference between the adult and preterm cells, but there was a significant difference between the newborn (0.30 sec−1) and adult (0.54 sec−1) cells. The percent shortening did not show any significant difference with age. Within the neonatal groups, there were no significant differences in morphometry, shortening or velocity. To facilitate comparison between ASM tissues of different sizes with different sized cells, we also expressed percent shortening and velocities relative to a hypothetical 1mm segment of tissue. Represented this way, the amount of shortening for all age groups was the same, but the predicted maximum velocity of the hypothetical preterm tissue (125–140 days) was significantly greater than for newborn.
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影响因子：
3.6
作者：
PENN, RB;WOLFSON, MR;SHAFFER, TH
通讯作者：
SHAFFER, TH
DOI：
10.1001/archpedi.1986.02140190059026
发表时间：
1986-05-01
影响因子：
--
作者：
BHUTANI, VK;RITCHIE, WG;SHAFFER, TH
通讯作者：
SHAFFER, TH
影响因子：
3.3
作者：
Meiss, RA
通讯作者：
Meiss, RA
影响因子：
3.3
作者：
MURPHY, TM;MITCHELL, RW;LEFF, AR
通讯作者：
LEFF, AR
影响因子：
3.3
作者：
PENN, RB;WOLFSON, MR;SHAFFER, TH
通讯作者：
SHAFFER, TH