Persistence of the viral genome in interferon-treated cells infected with oncogneic or nononcogenic viruses.
Persistence of the viral genome in interferon-treated cells infected with oncogneic or nononcogenic viruses.
复制标题
病毒基因组在感染致癌或非致癌病毒的干扰素处理细胞中持续存在。
DOI：
--
复制
发表时间：
1976
影响因子：
6.4
通讯作者：
E. Chang
中科院分区：
文献类型：
--
作者：
R. Friedman;J. Costa;J. Ramseur;M. Meyers;F. Jay;E. Chang
在长期感染鼠白血病病毒的AKR小鼠细胞中，用干扰素治疗9天导致持续抑制鼠白血病病毒的细胞外产生，但不抑制病毒细胞内p30抗原或逆转录酶。干扰素的去除导致这些作用的快速逆转。干扰素处理的小鼠L-细胞感染高多样性的水泡性口炎病毒或单纯疱疹病毒1型。感染性病毒和细胞内的病毒抗原被迅速消除从干扰素处理的培养物感染单纯疱疹病毒。在水泡性口炎病毒感染的培养物中，病毒滴度在干扰素处理的细胞中仍然很低，但大约两周后，病毒滴度迅速上升，培养物被破坏。如果在初次感染后9天重新开始干扰素治疗，则传染性水泡性口炎病毒被消除，并且没有证据表明病毒基因组在这些培养物中存活。在感染小鼠白血病病毒的培养物中，用干扰素处理可以抑制病毒的产生，但病毒基因组不能消除。然而，在水泡性口炎病毒或单纯疱疹病毒急性感染的细胞中，病毒基因组显然从干扰素处理的培养物中消除。
In AKR mouse cells chronically infected with a murine leukemia virus, treatment with interferon for nine days resulted in sustained inhibition of extracellular production of murine leukemia virus but no inhibition of viral intracellular p30 antigen or of reverse transcriptase. Removal of interferon resulted in rapid reversal of these effects. Interferon-treated mouse L-cells were infected with high multiplicities of vesicular stomatitis virus or herpes simplex virus type 1. Infectious virus and intracellular viral antigen were rapidly eliminated from the interferon-treated cultures infected with herpes simplex virus. In cultures infected with vesicular stomatitis virus, titers of virus remained low in interferon-treated cells, but after about two weeks they rose rapidly and the cultures were destroyed. If treatment with interferon was reinstituted as late as nine days after primary infection, infectious vesicular stomatitis virus was eliminated, and there was no evidence for survival of the viral genome in these cultures. In the cultures infected with murine leukemia virus, inhibition of production of virus by treatment with interferon was possible, but the viral genome was not eliminated. In cells acutely infected with vesicular stomatitis virus or herpes simplex virus, however, the viral genomes were apparently eliminated from cultures treated with interferon.