ESCRT, not intralumenal fragments, sorts ubiquitinated vacuole membrane proteins for degradation.
ESCRT, not intralumenal fragments, sorts ubiquitinated vacuole membrane proteins for degradation.
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DOI：
10.1083/jcb.202012104
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发表时间：
2021-08-02
期刊：
影响因子：
--
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Yang X;Reist L;Chomchai DA;Chen L;Arines FM;Li M
Yang等人应用了一种基于微流体的成像方法来解决关于泛素化溶酶体膜蛋白如何被分类到管腔中进行降解的争议。escrt负责分选，而不是腔内碎片。溶酶体（或真菌和植物中的液泡）是营养感知和细胞内稳态的重要细胞器。为了应对饥饿等环境压力，酵母液泡可以通过选择性地将膜蛋白内化到管腔中进行降解来调节其膜组成。关于选择性内部化机制，人们提出了两种相互竞争的模型。一种模型表明，ESCRT机制负责分类。相比之下，escrt独立的腔内片段（ILF）途径提出，由同型液泡融合产生的片段负责分选。在这里，我们应用了一种基于微流体的成像方法来捕捉体内的完整降解过程。结合活细胞成像和同步泛素化系统，我们证明了ILF货物不会通过腔内片段降解。相反，escrt在液泡膜上起作用，将它们分类到管腔中进行降解。我们进一步讨论了重建液泡膜蛋白降解的挑战。
Yang et al. applied a microfluidics-based imaging method to resolve a controversy regarding how ubiquitinated lysosome membrane proteins are sorted into the lumen for degradation. ESCRTs, but not intralumenal fragments, are responsible for the sorting. The lysosome (or vacuole in fungi and plants) is an essential organelle for nutrient sensing and cellular homeostasis. In response to environmental stresses such as starvation, the yeast vacuole can adjust its membrane composition by selectively internalizing membrane proteins into the lumen for degradation. Regarding the selective internalization mechanism, two competing models have been proposed. One model suggests that the ESCRT machinery is responsible for the sorting. In contrast, the ESCRT-independent intralumenal fragment (ILF) pathway proposes that the fragment generated by homotypic vacuole fusion is responsible for the sorting. Here, we applied a microfluidics-based imaging method to capture the complete degradation process in vivo. Combining live-cell imaging with a synchronized ubiquitination system, we demonstrated that ILF cargoes are not degraded through intralumenal fragments. Instead, ESCRTs function on the vacuole membrane to sort them into the lumen for degradation. We further discussed challenges in reconstituting vacuole membrane protein degradation.
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DOI：
10.1083/jcb.201611029
发表时间：
2017-10-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Oku M;Maeda Y;Kagohashi Y;Kondo T;Yamada M;Fujimoto T;Sakai Y
通讯作者：
Sakai Y
影响因子：
5.3
作者：
O'Donnell, Allyson F.;McCartney, Rhonda R.;Schmidt, Martin C.
通讯作者：
Schmidt, Martin C.
影响因子：
16.6
作者：
McNally, Erin Kate;Brett, Christopher Leonard
通讯作者：
Brett, Christopher Leonard
影响因子：
11.8
作者：
Karim，Mahmoud Abdul;McNally，Erin Kate;Brett，Christopher Leonard
通讯作者：
Brett，Christopher Leonard
影响因子：
7.7
作者：
Ivashov, Vasyl;Zimmer, Johannes;Teis, David
通讯作者：
Teis, David