Obligate gut symbiotic association in the sloe bug Dolycoris baccarum (Hemiptera: Pentatomidae)
Obligate gut symbiotic association in the sloe bug Dolycoris baccarum (Hemiptera: Pentatomidae)
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DOI：
10.1007/s13355-016-0453-0
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发表时间：
2017-02-01
影响因子：
1.3
通讯作者：
Kikuchi, Yoshitomo
中科院分区：
文献类型：
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作者：
Itoh, Hideomi;Matsuura, Yu;Kikuchi, Yoshitomo
许多植食性臭虫与其消化道中的特定细菌共生体有关。黑刺蝽（Linnaeus）是一种臭名昭著的农作物害虫，在中肠后部有许多囊状组织，称为隐窝，其中有一种特定的细菌共生体定植。在这里，我们通过组织分析、分子系统发育和特异引物组的诊断PCR来表征D. baccarum共生细菌。通过对细菌16S rRNA基因的克隆、测序和荧光原位杂交分析表明，该菌与中肠隐窝中的一种γ变形菌有关。分子系统发育分析表明，该共生体应归属于肠杆菌科泛菌属。诊断PCR和虫卵表面福尔马林消毒表明，臭虫通过涂抹虫卵垂直传播泛菌。绝育产生的异共生若虫死亡率高，没有成虫。此外，泛古菌的共生体在昆虫宿主外是不可培养的，这表明寄主-共生体的关系是专性的和亲密的。
A number of phytophagous stinkbugs are associated with specific bacterial symbionts in their alimentary tracts. The sloe bug Dolycoris baccarum (Linnaeus), a notorious pest of diverse crops, possesses a number of sac-like tissues, called crypts, in a posterior section of the midgut, wherein a specific bacterial symbiont colonizes. Here we characterized the symbiotic bacterium of D. baccarum by histological analysis, molecular phylogeny, and diagnostic PCR with a specific primer set. The cloning and sequencing analyses of bacterial 16S rRNA genes and fluorescent in situ hybridization demonstrated that the sloe bug is associated with a single species of Gammaproteobacteria in the midgut crypts. Molecular phylogenetic analysis strongly suggested that the symbiont should be placed in the genus Pantoea of the Enterobacteriaceae. Diagnostic PCR and egg surface sterilization with formalin indicated the stinkbug vertically transmits the Pantoea symbiont via egg-smearing. The sterilization-produced aposymbiotic nymphs showed high mortality and no insects reached adulthood. In addition, the Pantoea symbiont was uncultivable outside the insect host, indicating an obligate and intimate host-symbiont association.