Uptake of silica nanoparticles in the brain and effects on neuronal differentiation using different in vitro models
Uptake of silica nanoparticles in the brain and effects on neuronal differentiation using different in vitro models
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DOI：
10.1016/j.nano.2016.11.001
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发表时间：
2017-04-01
影响因子：
5.4
通讯作者：
Mevissen, Meike
中科院分区：
文献类型：
--
作者：
Ducray, Angelique D.;Stojiljkovic, Ana;Mevissen, Meike
纳米医学为脑部疾病的治疗提供了一个很有前途的工具，但它们可能与潜在的不良反应有关。本研究的目的是利用大鼠海马中分离的SH-SY5Y细胞和器官型切片培养物，研究用于激光组织焊接的二氧化硅纳米颗粒在大脑中的吸收情况。在海马培养物中，纳米颗粒主要被小胶质细胞吸收，但在分化的SH-SY5Y细胞中也发现了纳米颗粒。在原代海马细胞中摄取是时间和浓度依赖性的。透射电镜实验表明，在细胞质中存在纳米粒子聚集体和单粒子。在内质网中发现了纳米颗粒，但在其他细胞区室中没有发现。纳米颗粒暴露不损害细胞活力和神经炎症在原代海马培养在所有时间的调查。SH-SY5Y细胞的神经突生长没有明显改变，但神经元分化标志物表明纳米颗粒暴露后神经元分化诱导减少。(C) 2016 Elsevier Inc.版权所有。
Nanomedicine offers a promising tool for therapies of brain diseases, but they may be associated with potential adverse effects. The aim of this study was to investigate the uptake of silica-nanoparticles engineered for laser-tissue soldering in the brain using SH-SY5Y cells, dissociated and organotypic slice cultures from rat hippocampus. Nanoparticles were predominantly taken up by microglial cells in the hippocampal cultures but nanoparticles were also found in differentiated SH-SY5Y cells. The uptake was time-and concentration-dependent in primary hippocampal cells. Transmission electron microscopy experiments demonstrated nanoparticle aggregates and single particles in the cytoplasm. Nanoparticles were found in the endoplasmic reticulum, but not in other cellular compartments. Nanoparticle exposure did not impair cell viability and neuroinflammation in primary hippocampal cultures at all times investigated. Neurite outgrowth was not significantly altered in SH-SY5Y cells, but the neuronal differentiation markers indicated a reduction in neuronal differentiation induction after nanoparticle exposure. (C) 2016 Elsevier Inc. All rights reserved.