Activation of synovial fluid T lymphocytes by 60-kd heat-shock proteins in patients with inflammatory synovitis.
Activation of synovial fluid T lymphocytes by 60-kd heat-shock proteins in patients with inflammatory synovitis.
复制标题
炎症性滑膜炎患者中 60-kd 热休克蛋白激活滑液 T 淋巴细胞。
DOI：
10.1002/art.1780350107
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发表时间：
1992
影响因子：
--
通讯作者：
Gupta,RS
中科院分区：
文献类型：
--
作者：
Pope,RM;Lovis,RM;Gupta,RS
Objective.Synovial fluid lymphocytes from patients with rheumatoid arthritis and with other forms of inflammatory synovitis demonstrate enhanced proliferative responses toMycobacterium tuberculosisantigens, in particular, the 65‐kd heat‐shock protein. There is a high degree of homology between the human and the mycobacterial 60‐kd family of heat‐shock proteins. These studies were performed to determine if the enhanced response to the mycobacterial 65‐kd heat‐shock protein was due to cross‐reactivity of an immune response generated against the human homolog.Methods.These studies were performed by in vitro culture of isolated synovial fluid mononuclear cells with crude and purified antigens.Results.The synovial fluid lymphocytes of a majority of patients with rheumatoid arthritis recognized the mycobacterial 65‐kd heat‐shock protein, as evidenced by T cell proliferation. In contrast, only 18% of all samples tested responded to a highly purified recombinant human 60‐kd heat‐shock protein. With only one exception, proliferative responses to the mycobacterial antigen were stronger than those to the human homolog. The proliferative responses generated against mycobacterial 65‐kd heat‐shock proteins from different sources were highly correlated.Conclusion.The findings suggest that the enhanced proliferative response to the mycobacterial 65‐kd heat‐shock protein noted in most patients with rheumatoid arthritis and other forms of inflammatory synovitis is not due to cross‐reactivity of an immune response directed against the human heat‐shock protein.
