Chemical and physical properties of carbonated hydroxyapatite affect breast cancer cell behavior.
Chemical and physical properties of carbonated hydroxyapatite affect breast cancer cell behavior.
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DOI：
10.1016/j.actbio.2015.06.001
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发表时间：
2015-09
影响因子：
9.7
通讯作者：
Fischbach C
中科院分区：
文献类型：
--
作者：
Choi S;Coonrod S;Estroff L;Fischbach C
乳房微钙化是乳腺癌的常规乳房x线摄影检查，主要由非化学计量羟基磷灰石(Ca10-x(PO4)6-x(CO3)x(OH)2-x) （HA）组成。有趣的是，HA形态和碳酸盐取代在恶性和良性病变中有所不同。然而，这些变化是否(i)在功能上相互关联和（ii）影响恶性肿瘤仍不清楚，部分原因是缺乏允许评估这些可能性的模型系统。在这里，我们采用了96个基础良好的矿化培养平台来研究乳腺癌细胞的行为，以响应透明质酸化学和物理性质的系统变化。通过调节生长过程中模拟体液（SBF）溶液的碳酸盐含量，可以控制沉积HA的形态和碳酸盐取代。我们的研究结果表明，这些差异的联合效应和个体效应都改变了乳腺癌细胞的生长和致瘤性白细胞介素-8 （IL-8）的分泌。因此，碳酸钙掺入和形态的变化影响乳腺癌细胞的行为。总的来说，我们的数据强调了生物矿化培养平台对评估透明质酸材料特性对乳腺癌发病机制的功能贡献的重要性。
Breast microcalcifications are routinely explored for mammographic detection of breast cancer and primarily composed of non-stoichiometric hydroxyapatite (Ca10-x(PO4)6-x(CO3)x(OH)2-x) (HA). Interestingly, HA morphology and carbonate substitution vary in malignant vs. benign lesions. However, whether or not these changes (i) are functionally linked and (ii) impact malignancy remains unclear due in part to a lack of model systems that permit evaluating these possibilities. Here, we have adapted a 96 well-based mineralized culture platform to investigate breast cancer cell behavior in response to systematic changes in the chemical and physical properties of HA. By adjusting the carbonate content of the simulated body fluid (SBF) solutions used during growth, we can control the morphology and carbonate substitution of the deposited HA. Our results suggest that both the combined and individual effects of these differences alter breast cancer cell growth and secretion of tumorigenic interleukin-8 (IL-8). Consequently, changes in both HA carbonate incorporation and morphology impact the behavior of breast cancer cells. Collectively, our data underline the importance of biomineralized culture platforms to evaluate the functional contribution of HA materials properties to the pathogenesis of breast cancer.