Adsorption of dirhamnolipid on four microorganisms and the effect on cell surface hydrophobicity
Adsorption of dirhamnolipid on four microorganisms and the effect on cell surface hydrophobicity
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二鼠李脂对四种微生物的吸附及其对细胞表面疏水性的影响
DOI：
10.1007/s00253-007-1154-y
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发表时间：
2007-11-01
影响因子：
5
通讯作者：
Ren, Fang Yi
中科院分区：
文献类型：
--
作者：
Zhong, Hua;Zeng, Guang Ming;Ren, Fang Yi
本研究研究了二鼠李糖脂生物表面活性剂在革兰氏阴性铜绿假单胞菌、两种革兰氏阳性枯草芽孢杆菌和酵母解脂假丝酵母上的吸附，并讨论了吸附与细胞表面疏水性变化之间的因果关系。吸附不仅针对微生物，而且还取决于细胞的生理状态。不同鼠李糖基数或脂肪链长度的生物表面活性剂组分的吸附方式也表现出轻微的差异。这种吸附确实引起了细胞表面疏水性的规律性变化；然而，这些变化取决于所用鼠李糖脂溶液的浓度和吸附剂的生理条件。鼠李糖脂单体在细胞表面的取向和胶束沉积被认为是分别在低和高鼠李糖脂浓度下改变细胞疏水性的吸附的基本手段。这项研究提出了用低浓度生物表面活性剂改变细胞表面疏水性的可能性，这对于原位土壤修复可能具有重要意义。
In this study, adsorption of dirhamnolipid biosurfactant on a Gram-negativePseudomonas aeruginosa, two Gram-positiveBacillus subtilis, and a yeast,Candida lipolytica, was investigated, and the causality between the adsorption and change of cell surface hydrophobicity was discussed. The adsorption was not only specific to the microorganisms but also depended on the physiological status of the cells. Components of the biosurfactant with different rhamnosyl number or aliphatic chain length also exhibited slight difference in adsorption manner. The adsorption indeed caused the cell surface hydrophobicity to change regularly; however, the changes depended on both the concentrations of rhamnolipid solutions applied and the adsorbent physiological conditions. Orientation of rhamnolipid monomers on cell surface and micelle deposition are supposed to be the basic means of adsorption to change cell hydrophobicity at low and high rhamnolipid concentrations, respectively. This study proposed the possibility to modify cell surface hydrophobicity with biosurfactant of low concentrations, which may be of importance in in situ soil remediation.