Inhibition of DNA transcription using cationic mixed monolayer protected gold clusters
Inhibition of DNA transcription using cationic mixed monolayer protected gold clusters
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DOI：
10.1021/ja015556g
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发表时间：
2001-08-08
影响因子：
15
通讯作者：
Rotello, VM
中科院分区：
文献类型：
--
作者：
McIntosh, CM;Esposito, EA;Rotello, VM
DNA的有效识别是开发生物效应器的先决条件，生物效应器包括转录和翻译调节剂、转染载体和DNA传感器。为了提供一个有效的支架与DNA的多价相互作用，我们已经制造了混合单层保护的金簇（MMPC）功能化的四烷基铵配体，可以通过电荷互补性与DNA骨架相互作用。结合研究表明，MMPC和DNA形成一个电荷中和，非聚集的大会。控制这些组件的相互作用是非常有效的，完全抑制转录T7 RNA聚合酶在体外。
Efficient recognition of DNA is a prerequisite for the development of biological effectors, including transcription and translation regulators, transfection vectors, and DNA sensors. To provide an effective scaffold for multivalent interactions with DNA, we have fabricated mixed monolayer protected gold clusters (MMPCs) functionalized with tetraalkylammonium ligands that can interact with the DNA backbone via charge complementarity. Binding studies indicate that the MMPCs and DNA form a charge-neutralized, nonaggregated assembly. The interactions controlling these assemblies are highly efficient, completely inhibiting transcription by T7 RNA polymerase in vitro.