EFFECT OF A SHORT COURSE OF 1,25-DIHYDROXYVITAMIN-D3 ON BIOCHEMICAL MARKERS OF BONE REMODELING IN ADULT MALE-VOLUNTEERS
EFFECT OF A SHORT COURSE OF 1,25-DIHYDROXYVITAMIN-D3 ON BIOCHEMICAL MARKERS OF BONE REMODELING IN ADULT MALE-VOLUNTEERS
复制标题
DOI：
10.1016/8756-3282(91)90020-j
复制
发表时间：
1991-01-01
期刊：
影响因子：
4.1
通讯作者：
MOSEKILDE, L
中科院分区：
文献类型：
--
作者：
BOLLERSLEV, J;GRAM, J;MOSEKILDE, L
为了研究维生素D对骨重塑生化指标的刺激作用，15例正常男性（26-45岁，平均33.2岁）口服1,25-二羟基维生素D3，每日2 μ g，连续7天，随访16周。血清1,25-二羟维生素D3浓度在第一周内升高43% (p < 0.01)，而25-羟维生素D3水平无显著变化。血清免疫反应性甲状旁腺激素（iPTH）水平显著降低（p < 0.02），肾脏最大磷酸重吸收能力（TmP/GFR）显著升高（p < 0.05），提示维生素D水平升高对靶组织的影响。治疗周内血清磷酸盐和血清钙升高（p < 0.05），空腹肾磷和钙排泄量升高（p < 0.01）。然而，在观察期间羟基脯氨酸的排泄量逐渐下降。维生素D处理后第1周血清酸性磷酸酶活性升高，在第2周末达到显著水平（p < 0.05）。观察结束时，酸性磷酸酶活性仍显著升高（p < 0.02）。这些观察结果表明，新的骨吸收细胞的同步和募集。1,25-二羟基维生素D对成骨细胞生化标志物的直接反应是血清骨钙素（BGP）水平显著升高（p < 0.002），并在接下来的几周内逐渐下降。然而，在随访期间的最后两个月观察到第二次增加。维生素D治疗16周后，BGP仍显著升高（p < 0.01）。血清骨碱性磷酸酶活性逐渐升高（p < 0.0001）。由此可见，1,25-二羟基维生素D3短期治疗可刺激现有骨细胞，并能同步和激活骨重塑。然而，骨吸收细胞的激活并不会增加骨吸收。
To investigate the stimulatory effect of vitamin D on biochemical markers of bone remodeling, 15 normal men (aged 26-45 years, mean 33.2) were treated orally with 1,25-dihydroxyvitamin D3, 2-mu-g daily for 7 days, and followed for a total of 16 weeks. Serum concentrations of 1,25-dihydroxyvitamin D3 rose 43% during the first week (p < 0.01), with no significant alteration in the level of 25-hydroxyvitamin D3. Serum level of immunoreactive parathyroid hormone (1-84) (iPTH) decreased markedly (p < 0.02), and the maximal renal reabsorption capacity of phosphate (TmP/GFR) increased (p < 0.05), both indicating the impact of the raised vitamin D level on target tissues. Serum phosphate and serum calcium increased during the treatment week (p < 0.05), as did the fasting renal excretion of phosphate and calcium (p < 0.01). However, a gradual fall in the excretion of hydroxyproline was seen in the observation period. The serum activity of acid phosphatase increased in the first weeks after vitamin D treatment, reaching significance at the end of week 2 (p < 0.05). Acid phosphatase activity was still increased at the end of the observation period (p < 0.02). These observations suggest a syncronization and recruitment of new bone resorptive cells. The immediate response to 1,25-dihydroxyvitamin D administration on the biochemical markers of formative bone cells was a marked increase in the serum level of osteocalcin (BGP), (p < 0.002) with a gradually fall during the next weeks. A secondary increase, however, was observed in the last two months of the follow-up period. Sixteen weeks after initiation of vitamin D treatment, BGP was still markedly increased (p < 0.01). Moreover, a gradual increase in bone alkaline phosphatase activity in serum was observed (p < 0.0001). It is concluded that short-term treatment with 1,25-dihydroxyvitamin D3 stimulates existing bone cells, and is capable of syncronizing and activating bone remodeling. However, activation of bone resorptive cells is not followed by increased bone resorption.