INHIBITION OF ADIPOSE CONVERSION IN 3T3-L2 CELLS BY RETINOIC ACID
INHIBITION OF ADIPOSE CONVERSION IN 3T3-L2 CELLS BY RETINOIC ACID
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DOI：
10.1002/jss.400140214
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
RUSSELL, TR
中科院分区：
文献类型：
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作者：
MURRAY, T;RUSSELL, TR
研究了视黄酸在调节 3T3-L2 成纤维细胞分化为脂肪细胞中的作用。类维生素A能够有效抑制用1-甲基-3-异丁基黄嘌呤加地塞米松处理前脂肪细胞引起的脂肪转化程度。 10-6 M 视黄酸可抑制脂肪表型的形态学和酶学（脂肪酸合成酶活性）表达超过 90%。该抑制作用具有浓度依赖性，视黄酸水平低至 10-11 M 即可将脂肪转化减少 20%。视黄酸必须与引发剂同时施用才能有效。将非生长的前脂肪细胞暴露于视黄酸不会改变细胞响应甲基异丁基黄嘌呤加地塞米松的后续处理而分化的能力。该抑制是可逆的。通过添加视黄酸(10-6 M)阻断甲基异丁基黄嘌呤加地塞米松触发分化的培养物将很容易发生脂肪转化，以响应在不存在类维生素A的情况下用甲基异丁基黄嘌呤加地塞米松的第二次处理。当在补充有新生小牛血清或胎牛血清的培养基中用药物处理培养物时，发现了类似的分化抑制作用。在含有胎牛血清的培养基中，甲基异丁基黄嘌呤加地塞米松能够促进这些细胞分化的程度要大得多。
The role of retinoic acid in modulating the differentiation of 3T3-L2 fibroblasts into adipocytes was examined. The retinoid is capable of effectively inhibiting the degree of adipose conversion which is brought about by treatment of preadipocytes with 1-methyl-3-isobutylxanthine plus dexamethasone. Morhplogical and enzymatic (fatty acid synthetase activity) expression of the adipose phenotype are both inhibited more than 90% by 10-6 M retinoic acid. The inhibition is concentration dependent with retinoic acid levels as low as 10-11 M capable of reducing adipose conversion by 20%. Retinoic acid must be administered simultaneously with the triggering agents to be effective. Exposure of nongrowing preadipocytes to retinoic acid does not alter the ability of the cells to differentiate in response to a subsequent treatment with methylisobutylxanthine plus dexamethasone. The inhibition is reversible. Cultures in which methylisobutylxanthine plus dexamethasone triggered differentiation was blocked by addition of retinoic acid (10-6 M) will readily undergo adipose conversion in response to a 2nd treatment with methylisobutylxanthine plus dexamethasone in the absence of the retinoid. Similar inhibition of differentiation was found when cultures were treated with drugs in medium supplemented with either newborn calf serum or fetal calf serum. The extent to which methylisobutylxanthine plus dexamethasone are able to promote differentiation in these cells is considerably greater in medium containing fetal calf serum.