DNA repair capacity measured by high throughput alkaline comet assays in EBV-transformed cell lines and peripheral blood cells from cancer patients and healthy volunteers
DNA repair capacity measured by high throughput alkaline comet assays in EBV-transformed cell lines and peripheral blood cells from cancer patients and healthy volunteers
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DOI：
10.1016/j.mrgentox.2005.07.012
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发表时间：
2005-12-07
影响因子：
1.9
通讯作者：
Imai, T
中科院分区：
文献类型：
--
作者：
Iwakawa, M;Goto, M;Imai, T
我们收集了556例接受放射治疗的不同类型癌症患者和81名健康志愿者的外周血（PB）。我们将整个PB和Epstein-Barr病毒转化的淋巴母细胞样细胞系（EBLs）暴露于x射线（5 Gy）照射下。使用碱性彗星测定法，我们测量了立即DNA损伤，并在15分钟内测量了%残留损伤。在PB中，患者和健康志愿者的即时损伤相似，而乳腺癌（54.3 +/- A23.9）、宫颈癌（54.7 +/- A23.9）、头颈癌（56.8 +/- A24.4）、肺癌（60.1 +/- 23.5）和食管癌（59.5 +/- 33.7）患者的残余损伤%（平均+/- S.D.）显著高于健康供者（42.9 +/- 19.6）（P < 0.05）。我们在ebv转化的细胞系中没有观察到这种差异。因此，碱性彗星法测定的新鲜PB细胞的辐射敏感性与肿瘤状态有关。(c) 2005 Elsevier B.V.版权所有
We collected peripheral blood (PB) from 556 patients with various types of cancer who had undergone radiotherapy and from 81 healthy volunteers. We exposed whole PB and Epstein-Barr virus-transformed lymphoblastoid cell lines (EBLs) derived from the PB mononucleocytes to X-irradiation (5 Gy). Using the alkaline comet assay, we measured the immediate DNA damage and, at 15 min, the % residual damage. In PB, the immediate damage was similar in patients and healthy volunteers while the % residual damage (mean +/- S.D.) was significantly higher in patients with breast (54.3 +/- A23.9), cervical (54.7 +/- A23.9), head/neck (56.8 +/- A24.4), lung (60.1 +/- 23.5), or esophageal cancers (59.5 +/- 33.7) than in healthy donors (42.9 +/- 19.6) (P < 0.05). We did not observe such differences in the EBV-transformed cell lines. Thus, radiation sensitivity of fresh PB cells measured by the alkaline comet assay was related to cancer status. (c) 2005 Elsevier B.V. All rights reserved.