Norepinephrine-stimulated fatty-acid release and oxygen consumption in isolated hamster brown-fat cells. Influence of buffers, albumin, insulin and mitochondrial inhibitors.
Norepinephrine-stimulated fatty-acid release and oxygen consumption in isolated hamster brown-fat cells. Influence of buffers, albumin, insulin and mitochondrial inhibitors.
复制标题
去甲肾上腺素刺激离体仓鼠棕色脂肪细胞的脂肪酸释放和耗氧量。
DOI：
10.1111/j.1432-1033.1979.tb12948.x
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
O. Lindberg
中科院分区：
文献类型：
--
作者：
J. Nedergaard;O. Lindberg
从成年金黄仓鼠中分离的棕色脂肪细胞早期被发现对生理激动剂去甲肾上腺素的添加有反应，其具有增加的耗氧速率和脂肪酸释放。通过研究这些细胞，我们发现了以下情况。1.孵育介质（磷酸盐缓冲液）中存在白蛋白会增加去甲肾上腺素诱导的脂肪酸释放，并趋于稳定耗氧速率;在空气中用5% CO2鼓泡磷酸盐缓冲液对脂肪酸释放仅有轻微影响。2.在存在白蛋白的情况下，去甲肾上腺素诱导的耗氧速率在碳酸氢盐缓冲液中也是稳定的;它高于磷酸盐+ CO2缓冲液，并且棕色脂肪细胞对去甲肾上腺素具有更高的敏感性。3. 20 mM磷酸盐（如例如存在于磷酸盐缓冲液中）抑制脂肪酸释放和氧消耗。4.胰岛素抑制氧消耗的速率，但仅在去甲肾上腺素的次优浓度下。5.辛酸盐抑制次最大去甲肾上腺素诱导的呼吸，表明去甲肾上腺素刺激的棕色脂肪细胞中发生了一些氧化磷酸化。6.棕色脂肪的脂肪酸输出应被视为生理上重要的。
Brown fat cells isolated from adult golden hamsters have earlier been found to respond to addition of the physiological agonist norepinephrine with an increased rate of oxygen consumption and with fatty acid release. Working with these cells, we found the following. 1. The presence of albumin in the incubation medium (phosphate buffer) increases norepinephrine-induced fatty acid release and tends to stabilize the rate of oxygen consumption; bubbling of phosphate buffer with 5% CO2 in air has only a slight effect on fatty acid release. 2. In the presence of albumin, the norepinephrine-induced rate of oxygen consumption is also stable in bicarbonate buffer; it is higher than in the phosphate + CO2 buffer and the brown fat cells have a higher sensitivity to norepinephrine. 3. 20 mM phosphate (as e.g. present in a phosphate buffer) inhibits both fatty acid release and oxygen consumption. 4. Insulin inhibits the rate of oxygen consumption, but only at suboptimal concentrations of norepinephrine. 5. Atractylate inhibits submaximal norepinephrine-induced respiration, indicating that some oxidative phosphorylation takes place in norepinephrine-stimulated brown fat cells. 6. Fatty acid export from brown fat should be regarded as physiologically important.