Expression feature of CD3, FcεRIγ, and Zap-70 in patients with chronic lymphocytic leukemia
Expression feature of CD3, FcεRIγ, and Zap-70 in patients with chronic lymphocytic leukemia
复制标题
DOI：
10.1179/102453312x13221316477895
复制
发表时间：
2012-03-01
期刊：
影响因子：
1.9
通讯作者：
Li, Yangqiu
中科院分区：
文献类型：
--
作者：
Huang, Le;Chen, Shaohua;Li, Yangqiu
在白血病患者中，T细胞功能随着疾病的进展而受到抑制。慢性淋巴细胞白血病（CLL）患者都存在一定程度的免疫缺陷。为了阐明CLL中T细胞受体信号转导的特征，分析了外周血单个核细胞（PBMC）中CD 3 γ、δ、β和ζ链、Fc β RI γ和Zap-70基因的表达水平。应用SYBR绿色荧光定量聚合酶链反应（PCR）技术检测13例慢性淋巴细胞白血病（CLL）患者和13例健康人外周血单个核细胞（PBMCs）基因表达水平，并以10例B细胞急性淋巴细胞白血病（B-ALL）为对照。β 2-微球蛋白基因用作内源性参考。通过使用2-（Delta Ct）x 100%方法分析基因的相对mRNA表达水平。在CLL样本中发现CD 3 γ、CD 4和zeta链基因以及Fc γ RI γ基因的表达水平显著较低。此外，CD 3 zeta和Fc ε RI γ基因之间的表达水平失去了负相关性。CLL中Zap-70的表达水平低于健康对照组，但高于B-ALL组。CD 3 zeta和Zap-70基因的表达水平在健康组和CLL组中均无显著相关性。总之，这些结果提供了CLL中CD 3 γ、δ、β和ζ链以及CD 3 ζ相关基因Fc γ RI γ和Zap-70的全球基因表达谱。这些基因表达水平的缺乏可能代表了与T细胞免疫缺陷相关的特征。该研究可能有助于更好地了解CLL患者的细胞免疫特征。
In leukemia patients, T-cell function has been suppressed with the disease progress. Patients with chronic lymphocytic leukemia (CLL) are all to a degree immunodeficient. In order to elucidate the feature of T-cell receptor signal transduction in CLL, the expression levels of CD3 gamma, delta, epsilon, and zeta chain, Fc epsilon RI gamma, and Zap-70 genes in peripheral blood mononuclear cells (PBMCs) were analyzed. Real-time polymerase chain reaction with SYBR Green technique was used for detecting the gene expression level in PBMCs from 13 patients with CLL, 13 healthy individuals, and 10 B-cell acute lymphocytic leukemia (B-ALL) served as control. The beta 2-microglobulin gene was used as an endogenous reference. Relative mRNA expression level of genes was analyzed by using the 2-(Delta Ct) x 100% method. Significant lower expression levels of CD3 gamma, epsilon, and zeta chain genes, as well as Fc epsilon RI gamma gene were found in CLL samples. Moreover, there was lost the negative correlation of the expression levels between CD3 zeta and Fc epsilon RI gamma genes. The expression level of Zap-70 in CLL was lower than those from healthy controls, while higher than those from B-ALL group. There was no significant correlation between the expression levels of CD3 zeta and Zap-70 genes neither in the healthy group nor in the CLL group. In conclusion, the results provide a global gene expression profile of CD3 gamma, delta, epsilon, and zeta chains, and the CD3 zeta-related genes Fc epsilon RI gamma and Zap-70 in CLL. Deficiency of these gene expression levels might represent the feature related to T-cell immunodeficiency. The study might contribute to better understand the cellular immune features in CLL patients.