Assessing resolution in super-resolution imaging
Assessing resolution in super-resolution imaging
复制标题
DOI：
10.1016/j.ymeth.2015.07.001
复制
发表时间：
2015-10-15
期刊：
影响因子：
4.8
通讯作者：
Dobbie, Ian M.
中科院分区：
文献类型：
--
作者：
Demmerle, Justin;Wegel, Eva;Dobbie, Ian M.
分辨率是所有成像领域的核心概念，特别是在光学显微镜中，但它很容易被误解。光学分辨率的数学定义由阿贝编纂，并在世纪后期由瑞利判据实际定义。在这一时期，传统分辨率的极限也达到了，人们认为物理学的基本限制阻止了分辨率的进一步提高。随着近年来一系列超分辨率技术的发展，有必要重新审视光学分辨率的概念。超分辨率模式的根本差异意味着分辨率不是技术之间直接可转移的度量。本文考虑了这些新技术提出的分辨率问题，并提出了不同的超分辨率方法之间的分辨率比较的方法。(C)由Elsevier Inc.出版。
Resolution is a central concept in all imaging fields, and particularly in optical microscopy, but it can be easily misinterpreted. The mathematical definition of optical resolution was codified by Abbe, and practically defined by the Rayleigh Criterion in the late 19th century. The limit of conventional resolution was also achieved in this period, and it was thought that fundamental constraints of physics prevented further increases in resolution. With the recent development of a range of super-resolution techniques, it is necessary to revisit the concept of optical resolution. Fundamental differences in super-resolution modalities mean that resolution is not a directly transferrable metric between techniques. This article considers the issues in resolution raised by these new technologies, and presents approaches for comparing resolution between different super-resolution methods. (C) 2015 Published by Elsevier Inc.