Synergistic effects of some plant growth regulators on in vitro shoot proliferation of korarima (Aframomum corrorima (Braun) Jansen)
Synergistic effects of some plant growth regulators on in vitro shoot proliferation of korarima (Aframomum corrorima (Braun) Jansen)
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DOI：
10.5897/ajb2006.000-5082
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发表时间：
2006-10
影响因子：
--
通讯作者：
W. Tefera;S. Wannakrairoj
中科院分区：
文献类型：
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作者：
W. Tefera;S. Wannakrairoj
研究了几种植物生长调节剂对埃塞俄比亚一种重要的食用和药用植物Korarima（Corromomum corrorima（Braun）Jansen）的芽增殖和生长的协同效应。使用添加有5%椰子水（CW）的Murashige和Skoog（1962）（MS）培养基从根茎的腋芽外植体开始培养。0.5mg/l的噻苯隆（TDZ）与3 mg/l的多效唑（PBZ）组合使用在8周时间内产生约26个芽/外植体（约为对照的12.6倍）。当TDZ在0.5mg/l时，与2 mg/l的抑霉唑（IMA）或3 mg/l的N6-苄基腺嘌呤（BA）在培养基中同时使用时，芽增殖也被增强。随后的芽伸长和功能性根的发展后，达到一至三个月的继代培养的植物生长调节剂（PGR）无基础培养基。本研究所建立的方法可用于大规模的A. corrorima通过组织培养。
The synergistic effects of some plant growth regulators was investigated upon shoot proliferation and growth of korarima (Aframomum corrorima (Braun) Jansen), an important culinary and medicinal plant species native to Ethiopia. Cultures were initiated from axillary bud explants of rhizome using Murashige and Skoog (1962) (MS) medium added with 5% coconut water (CW). The use of 0.5 mg/l thidiazuron (TDZ) in combination with 3 mg/l paclobutrazol (PBZ) gave about 26 shoots/explant (about 12.6-fold than the control) within eight weeks time. Shoot multiplication was also enhanced when TDZ at 0.5 mg/l was simultaneously used with either 2 mg/l imazalil (IMA) or 3 mg/l N6-benzyladenine (BA) in the culture medium. Subsequent shoot elongation and development of functional roots was attained after one to three monthly-subcultures on a plant growth regulator (PGR)-free basal medium. The protocol developed from the present study could be used for the large-scale multiplication of A. corrorima by tissue culture.