Amino acid racemization and the preservation of ancient DNA
Amino acid racemization and the preservation of ancient DNA
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DOI：
10.1126/science.272.5263.864
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发表时间：
1996-05-10
期刊：
影响因子：
56.9
通讯作者：
Paabo, S
中科院分区：
文献类型：
--
作者：
Poinar, HN;Hoss, M;Paabo, S
天冬氨酸、丙氨酸和亮氨酸的外消旋程度为评估古组织样本是否含有内源DNA提供了标准。在天冬氨酸D/L比值超过0.08的样本中，无法检索古DNA序列。据称有数百万年历史的古生物学发现显示出广泛的外消旋化，并且存在的氨基酸主要是污染物，例外的是保存在琥珀中的一些昆虫的氨基酸。
The extent of racemization of aspartic acid, alanine, and leucine provides criteria for assessing whether ancient tissue samples contain endogenous DNA, In samples in which the D/L ratio of aspartic acid exceeds 0.08, ancient DNA sequences could not be retrieved. Paleontological finds from which DNA sequences purportedly millions of years old have been reported show extensive racemization, and the amino acids present are mainly contaminates, An exception is the amino acids in some insects preserved in amber.