Efficacy of Alaska Pollock Gelatin Sealant for Pulmonary Air Leakage in Porcine Models
Efficacy of Alaska Pollock Gelatin Sealant for Pulmonary Air Leakage in Porcine Models
复制标题
阿拉斯加狭鳕明胶密封剂对猪模型肺漏气的疗效
DOI：
10.1016/j.athoracsur.2021.05.023
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Sato Yukio
中科院分区：
文献类型：
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作者：
Yanagihara Takahiro;Maki Naoki;Wijesinghe A.I.;Sato Shoko;Saeki Yusuke;Kitazawa Shinsuke;Yamaoka Masatoshi;Kobayashi Naohiro;Kikuchi Shinji;Goto Yukinobu;Ichimura Hideo;Watanabe Shiharu;Taguchi Tetsushi;Sato Yukio
背景肺切除术后长期漏气是一种常见的并发症。我们已经开发了一种新的密封剂的基础上，疏水改性的阿拉斯加鳕鱼衍生的明胶（ApGltn）密封剂。本研究的目的是评估的ApGltn密封剂的粘合强度相比，纤维蛋白密封剂使用一种新的喷雾系统在体外和体内modelsMethodsPleural缺陷在体外和体内猪模型，其中ApGltn密封剂或纤维蛋白密封剂被施加。通过逐步增加气道压力来评估耐压性，以确认密封部位的漏气。结果在离体实验中，ApGltn密封剂的泄漏压力明显大于纤维蛋白密封剂（分别为102.94 ± 15.6 cm H2O和28.37 ± 5.1 cm H2O）（P<0.01）。在体内实验中，ApGltn封闭剂的泄漏压力也显著大于纤维蛋白封闭剂（分别为68.82 ± 18.04 cm H2O和43.33 ± 7.13 cm H2O）（P= 0.043）。组织学检查证实，ApGltn密封剂紧紧地粘附到胸膜和胸膜defect.ConclusionsThe表面ApGltn密封剂具有足够高的粘附质量在离体和体内猪肺，这可以被认为是合适的和有效的用于预防从肺漏气。
BackgroundPostoperative prolonged air leakage is a frequent complication after lung resection. We have developed a new sealant based on a hydrophobically modified Alaska pollock-derived gelatin (ApGltn) sealant. The purpose of this study was to evaluate the adhesive strength of the ApGltn sealant in comparison with a fibrin sealant using a new spray system in ex vivo and in vivo models.MethodsPleural defects in ex vivo and in vivo porcine models were created, to which the ApGltn sealant or the fibrin sealant was applied. The pressure resistance was assessed with a stepwise increase in airway pressure to confirm air leakage from the sealing site. Tissue samples covered with each sealant were obtained for histologic assessment.ResultsIn the ex vivo experiment, the leak pressures of the ApGltn sealant were significantly greater than those of the fibrin sealant (102.94 ± 15.6 cm H2O and 28.37 ± 5.1 cm H2O, respectively) (P< .01). In the in vivo experiment, the leak pressures of the ApGltn sealant were also significantly greater than those of the fibrin sealant (68.82 ± 18.04 cm H2O and 43.33 ± 7.13 cm H2O, respectively) (P= .043). The histologic examination confirmed that the ApGltn sealant adhered tightly to both the pleura and the surface of the pleural defect.ConclusionsThe ApGltn sealant has sufficiently high adhesive quality in ex vivo and in vivo porcine lungs, which could be considered suitable and effective for use in the prevention of air leakage from the lungs.