Can zebrafish be used as a model to study the neurodevelopmental causes of autism?
Can zebrafish be used as a model to study the neurodevelopmental causes of autism?
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DOI：
10.1034/j.1601-183x.2003.00038.x
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发表时间：
2003-10
期刊：
影响因子：
3.5
通讯作者：
V. Tropepe;H. Sive
中科院分区：
文献类型：
--
作者：
V. Tropepe;H. Sive
斑马鱼已被证明是分析脊椎动物发育问题的优秀模型。在这篇综述中，我们询问斑马鱼是否是分析自闭症神经发育原因的可行模型。在回答这个问题时，需要考虑三个主题。首先，讨论了斑马鱼作为模型的一般属性，包括低成本维护、快速生命周期和多种可用技术。这些技术包括大规模遗传筛选、靶向功能丧失和获得方法以及胚胎学检测。其次，我们考虑斑马鱼和哺乳动物大脑发育、结构和功能的保护。第三，我们讨论了斑马鱼作为人类疾病模型的令人印象深刻的用途，并提出了几种可以利用斑马鱼来剖析自闭症遗传基础的策略。我们的结论是，斑马鱼系统可以为理解自闭症做出重要贡献。
The zebrafish has proven to be an excellent model for analyzing issues of vertebrate development. In this review we ask whether the zebrafish is a viable model for analyzing the neurodevelopmental causes of autism. In developing an answer to this question three topics are considered. First, the general attributes of zebrafish as a model are discussed, including low cost maintenance, rapid life cycle and the multitude of techniques available. These techniques include large‐scale genetic screens, targeted loss and gain of function methods, and embryological assays. Second, we consider the conservation of zebrafish and mammalian brain development, structure and function. Third, we discuss the impressive use of zebrafish as a model for human disease, and suggest several strategies by which zebrafish could be used to dissect the genetic basis for autism. We conclude that the zebrafish system could be used to make important contributions to understanding autistic disorders.