Inhibition of Free DNA Degradation by the Deformation of DNA Exposed to Trace Polycyclic Aromatic Hydrocarbon Contaminants
Inhibition of Free DNA Degradation by the Deformation of DNA Exposed to Trace Polycyclic Aromatic Hydrocarbon Contaminants
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通过暴露于痕量多环芳烃污染物的 DNA 变形来抑制游离 DNA 降解
DOI：
10.1021/es103215b
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发表时间：
2010-12-01
影响因子：
11.4
通讯作者：
Wang, Qian
中科院分区：
文献类型：
--
作者：
Kang, Fuxing;Gao, Yanzheng;Wang, Qian
通过常规光谱分析和微量滴定检测多环芳烃 (PAH) 对 DNA 降解的快速抑制作用。目的是确定PAHs是否抑制DNA酶I对游离DNA的降解。结果表明，模型PAHs菲和芘与游离DNA结合，减缓DNA酶I对DNA的降解，菲诱导的抑制作用强于芘。痕量 PAH 不会导致 DNase I 失活。 PAH 暴露后，DNase I 酶在与酰胺 II 和 III 相关的红外吸收带中仅表现出轻微的变化，并且在其他带中没有观察到 Mange。 DNA 的降解减速归因于插入双链的 PAH 引起的 DNA 结构、主链组成和鸟嘌呤成分的变化，以及咪唑环与芘结合产生的咪唑类衍生物。
A rapid inhibitory effect of polycyclic aromatic hydrocarbons (PAHs) on DNA degradation was examined by conventional spectral analysis and microtitration. The purpose was to determine whether PAHs inhibited free DNA degradation by the enzyme DNase I. The results showed that model PAHs phenanthrene and pyrene combined with free DNA to decelerate DNA degradation by DNase I. Phenanthrene-induced inhibition was stronger than that of pyrene. Trace level of PAHs did not induce DNase I deactivation. The DNase I enzyme exhibited only slight shifts in IR absorption bands related to amide II and III upon PAH exposure, and no :Mange was observed with other bands. The decelerating degradation of DNA is attributed to the changes in structure, backbone composition, and guanine constituents of DNA induced by PAHs inserted into double strands, and to the imidazole-like derivates from the combination of imidazole rings with pyrene.