Clavatol and patulin formation as the antagonistic principle of Aspergillus clavatonanicus, an endophytic fungus of Taxus mairei
Clavatol and patulin formation as the antagonistic principle of Aspergillus clavatonanicus, an endophytic fungus of Taxus mairei
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棒曲醇和棒曲霉素的形成是红豆杉内生真菌棒曲霉的拮抗原理
DOI：
10.1007/s00253-008-1371-z
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发表时间：
2008-04-01
影响因子：
5
通讯作者：
Lin, Fu-Cheng
中科院分区：
文献类型：
--
作者：
Zhang, Chu-Long;Zheng, Bi-Qiang;Lin, Fu-Cheng
许多植物内生真菌具有保护植物免受病原菌侵害的作用，但其拮抗机制尚不清楚。在这项研究中，我们希望了解内生曲霉属，分离自南方红豆杉的曲霉是否真的会产生生物活性成分，以及如果是，（a）它们是否会拮抗植物病原真菌;和（B）该曲霉是否也会在与这些真菌对抗的条件下产生该化合物。从T.通过形态特征和rDNA内转录间隔区（ITS rDNA）序列分析，初步鉴定该菌株为棒曲霉（Aspergillus clavatonanicus）。当在表面培养物中生长时，真菌产生棒曲霉醇（2 '，4'-二羟基-3 '，5'-二甲基苯乙酮）和棒曲霉素（2-羟基-3，7-二氧杂双环[4.3.0]壬-5，9-二烯-8-酮），如通过NMR、MS、X-射线和EI-MS分析所示。这两种化合物在体外均表现出对几种植物病原真菌的抑制活性，灰葡萄孢（Botrytis cinerea）、灰葡萄孢（Didymella bryophylla）、尖孢镰刀菌（Fusarium oxysporum f.）黄瓜丝核菌（Rhizoctonia solani）和终极腐霉（Pythium ultimum）。在与P. ultimum对峙过程中，A. clavatonanicus拮抗其对终极青霉的生长，并且棒曲霉素和棒曲霉素都作为仅有的生物活性组分形成，尽管具有不同的动力学。我们的结论是A. clavatonanicus产生棒曲霉醇和棒曲霉素，这两种聚酮化合物可能参与了T. mairei抵抗植物病原体的攻击。
Many endophytic fungi are known to protect plants from plant pathogens, but the antagonistic mechanism has rarely been revealed. In this study, we wished to learn whether an endophytic Aspergillus sp., isolated from Taxus mairei, would indeed produce bioactive components, and if so whether (a) they would antagonize plant pathogenic fungi; and (b) whether this Aspergillus sp. would produce the compound also under conditions of confrontation with these fungi. The endophytic fungal strain from T. mairei was identified as Aspergillus clavatonanicus by analysis of morphological characteristics and the sequence of the internal transcribed spacers (ITS rDNA) of rDNA. When grown in surface culture, the fungus produced clavatol (2',4'-dihydroxy-3',5'-dimethylacetophenone) and patulin (2-hydroxy-3,7-dioxabicyclo [4.3.0]nona-5,9-dien-8-one), as shown by shown by NMR, MS, X-ray, and EI-MS analysis. Both exhibited inhibitory activity in vitro against several plant pathogenic fungi, i.e., Botrytis cinerea, Didymella bryoniae, Fusarium oxysporum f. sp. cucumerinum, Rhizoctonia solani, and Pythium ultimum. During confrontation with P. ultimum, A. clavatonanicus antagonized its growth of P. ultimum, and both clavatol as well as patulin were formed as the only bioactive components, albeit with different kinetics. We conclude that A. clavatonanicus produces clavatol and patulin, and that these two polyketides may be involved in the protection of T. mairei against attack by plant pathogens by this Aspergillus sp.