The lung cancer breath signature: a comparative analysis of exhaled breath and air sampled from inside the lungs.
The lung cancer breath signature: a comparative analysis of exhaled breath and air sampled from inside the lungs.
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DOI：
10.1038/srep16491
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发表时间：
2015-11-12
影响因子：
4.6
通讯作者：
D'Amico A
中科院分区：
文献类型：
--
作者：
Capuano R;Santonico M;Pennazza G;Ghezzi S;Martinelli E;Roscioni C;Lucantoni G;Galluccio G;Paolesse R;Di Natale C;D'Amico A
20多年的研究结果表明，呼出的挥发性有机化合物与肺癌之间存在联系。然而，这些化合物的来源仍然没有完全阐明。尽管简单地认为肺中的癌组织直接将挥发性代谢物排放到气道中，但一些论文指出代谢物被血液收集，然后在肺中的空气-血液界面交换。为了阐明这一主题，我们进行了一项实验，用一个改良的支气管镜探头收集两个肺内的呼吸和空气。样品用气相色谱-质谱联用仪（GC-MS）和电子鼻进行测定。我们发现，电子鼻的诊断能力并不取决于样本中是否存在癌症，在这两种情况下，癌症和非癌症样本之间的正确分类率均达到90%以上。另一方面，GC-MS的多变量分析在两个肺之间实现了仅76%的正确分类率。从肺中取样的呼吸和空气的GC-MS分析表明，从肺内部到呼出气的VOC模式基本上得到了保留。
Results collected in more than 20 years of studies suggest a relationship between the volatile organic compounds exhaled in breath and lung cancer. However, the origin of these compounds is still not completely elucidated. In spite of the simplistic vision that cancerous tissues in lungs directly emit the volatile metabolites into the airways, some papers point out that metabolites are collected by the blood and then exchanged at the air-blood interface in the lung. To shed light on this subject we performed an experiment collecting both the breath and the air inside both the lungs with a modified bronchoscopic probe. The samples were measured with a gas chromatography-mass spectrometer (GC-MS) and an electronic nose. We found that the diagnostic capability of the electronic nose does not depend on the presence of cancer in the sampled lung, reaching in both cases an above 90% correct classification rate between cancer and non-cancer samples. On the other hand, multivariate analysis of GC-MS achieved a correct classification rate between the two lungs of only 76%. GC-MS analysis of breath and air sampled from the lungs demonstrates a substantial preservation of the VOCs pattern from inside the lung to the exhaled breath.