A broad amplification pattern at 3q in squamous cell lung cancer -: A fluorescence in situ hybridization study
A broad amplification pattern at 3q in squamous cell lung cancer -: A fluorescence in situ hybridization study
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DOI：
10.1016/s0165-4608(99)00146-6
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发表时间：
2000-02-01
影响因子：
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通讯作者：
Knuutila, S
中科院分区：
文献类型：
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作者：
Kettunen, E;El-Rifai, W;Knuutila, S
在肺鳞状细胞癌（SQCC）中，3q的DNA拷贝数频繁增加，3q 24-qter的重叠区域最小，这表明3q基因在SQCC肿瘤发生中的重要性。为了进一步表征在3q的DNA序列的增益，我们进行了间期荧光原位杂交（FISH）分析16石蜡包埋的SQCC肿瘤样本，以前已经研究了比较基因组杂交（CGH）。11个酵母人工染色体（YAC）探针位于3q 25类似于q27和3号染色体特异性着丝粒探针Mere用于分析。所有SQCC肿瘤均显示出使用9-11探针的DIVA序列拷贝数增加。在5个肿瘤（31%）中，着丝粒信号的数量从3到5不等，YAC/着丝粒信号比为1.0，表明在这些病例中，3q处DNA序列拷贝数的增加是由3号染色体的多体性引起的。在11个肿瘤（69%）中，YAC/着丝粒信号比在1.5和4.7之间变化，表明DNA序列拷贝数的增加是由于3q处的DNA序列的染色体内获得。在每种情况下，几个YAC显示信号数量增加，表明获得的面积相对较大。因此，我们的研究结果表明，位于3q 25类似于q27的多个基因参与了SQCC的肿瘤发生。(C)Elsevier Science Inc.，2000. All rights reserved.
Frequent DNA copy number gain at 3q, with minimal overlapping area at 3q24-qter, has previously been reported in squamous cell carcinoma of the lung (SQCC), implicating the importance of genes at 3q in the tumorigenesis of SQCC. To further characterize the gain of DNA sequences at 3q, we performed interphase fluorescence in situ hybridization (FISH) analysis on 16 paraffin-embedded SQCC tumor samples that had previously been studied by comparative genomic hybridization (CGH). Eleven yeast artificial chromosome (YAC) probes located at 3q25 similar to q27 and a chromosome 3-specific centromeric probe Mere used in the analysis. All SQCC tumors showed increase in DIVA sequence copy number with 9-11 probes. In 5 tumors (31%) the number of centromeric signals varied from 3 to 5 and the YAC/centromeric signal ratio was 1.0, suggesting that the increase in DNA sequence copy number at 3q in these cases resulted from polysomy of chromosome 3. In 11 tumors (69%), the YAC/centromeric signal ratio varied between 1.5 and 4.7, indicating that the increase in DNA sequence copy number was due to intrachromosomal gain of DNA sequences at 3q. In each case, several YACs showed increased number of signals, demonstrating that the gained area was relatively large. Our findings therefore suggest that multiple genes located at 3q25 similar to q27 are involved in the tumorigenesis of SQCC. (C) Elsevier Science Inc., 2000. All rights reserved.