La autoantigen suppresses IRES-dependent translation of the hepatitis A virus
La autoantigen suppresses IRES-dependent translation of the hepatitis A virus
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DOI：
10.1016/j.bbrc.2008.01.163
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发表时间：
2008-04-18
影响因子：
3.1
通讯作者：
Gauss-Mueller, Verena
中科院分区：
文献类型：
--
作者：
Cordes, Susann;Kusov, Yuri;Gauss-Mueller, Verena
人rna结合蛋白La是典型的小核糖核酸病毒脊髓灰质炎病毒ires依赖性翻译起始过程中必不可少的反式作用因子。甲型肝炎病毒（HAV）是该病毒家族的一种不寻常的成员，宿主蛋白在其低效率翻译和缓慢复制中的作用尚不清楚。利用体内小干扰RNA和体外纯化的La，我们首次证明La抑制HAV ires介导的翻译和复制。我们发现La特异性地结合到HAV IRES的不同部分，并且与脊髓灰质炎病毒不同，HAV蛋白酶3C不会切割La。la介导的HAV翻译的抑制和脊髓灰质炎病毒翻译的刺激暗示了病毒TRES元件之间意想不到的机制差异。(C) 2008爱思唯尔公司版权所有。
The human RNA-binding protein La, is an essential trans-acting factor in IRES-dependent translation initiation of poliovirus, the prototypic picornavirus. For hepatitis A virus (HAV), an unusual member of this virus family, the role of host proteins in its inefficient translation and slow replication is unclear. Using small interfering RNA in vivo and purified La in vitro, we demonstrate for the first time that La suppresses HAV IRES-mediated translation and replication. We show that La binds specifically to distinct parts of the HAV IRES and that-unlike poliovirus-HAV proteinase 3C does not cleave La. The La-mediated suppression of HAV translation and stimulation of poliovirus translation implies unexpected mechanistic differences between viral TRES elements. (C) 2008 Elsevier Inc. All rights reserved.